safety guidelines for laboratory

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safety guidelines for laboratory

1.0 Purpose
1.1 To describe a procedure for Safety guidelines for Laboratory.

2.0 Scope
2.1 This standard operating procedure is applicable for Safety guidelines for Laboratory in QC Laboratory at ABC Pvt. Ltd.

3.0 Responsibility
Officer QC/ Executive QC Preparation of SOP
Assistant Manager Reviewing and Training of SOP
Manager QC Implementation of SOP
4.0 Accountability
4.1 Manager Quality Control is accountable for implementation and compliance with SOP.

5.0 Procedure
5.1 General Safety Guidelines
5.1.1 Always wear proper eye protection in chemical work, handling and storage areas.
5.1.2 Always know the physical and chemical hazards associated with the materials that are being utilized in the lab.
5.1.3 Each employee is to wear a clean protective gown, while in the laboratory.
5.1.4 Confine long hair and loose clothing. Do not wear high-heeled shoes, open-toed shoes,

sandals or shoes made of woven material. Do not wear shorts, cutoffs or miniskirts,

or cover these with a suitable lab coat or apron.
5.1.5 No sitting on laboratory work benches is permitted. Personal articles are not to

be placed on working surfaces; they should be stored in individual lockers.
5.1.6 Always wash hands and arms with soap and water before leaving the work area.

This applies even if you have been wearing gloves.
5.1.7 Never perform any hazardous work when alone in the laboratory. At least two

people must be present and undergraduate students must be supervised by an instructor at all times.
5.1.8 Never perform unauthorized work, preparations or experiments.
5.1.9 Never engage in horseplay, pranks or other acts of mischief in chemical or biological work areas.
5.1.10 Never remove chemicals from the facility without proper authorization.
5.1.11 Be familiar with the location of emergency equipment – fire alarm, fire extinguisher,

emergency eyewash and safety shower. Know the appropriate emergency response procedures.
5.1.12 Use equipment and hazardous chemicals only for their intended purposes.
5.1.13 Never mouth pipette chemicals when transferring solutions. Instead, you should

always use a pipette bulb to transfer solutions
5.1.14 Never return reagents to stock bottles.
5.1.15 Each individual is expected to conduct themselves in a professional manner.
5.1.16 Always lubricate glass tubing thermometers, or thistle tubes before inserting them into a stopper.
5.1.17 Use a hood whenever there is a possibility of poisonous or irritating fumes being

emitted from the chemicals being utilized.
5.1.18 Never leave an experiment unattended while it is being heated or is rapidly reacting.
5.1.19 Keep equipment back from the edge of the lab bench to prevent spillage.
5.1.20 Support all beakers and flasks with clamps. Do not use cracked or chipped glassware.
5.1.21 Never use flames with volatile solvents.
5.1.22 Read all labels on chemicals twice before using them in the lab.
5.1.23 Report any accident, however minor, Quality Control Manager or lab supervisor immediately.
5.1.24 Maintain laboratory facilities and equipment in a safe operating condition.
5.1.25 Ensure that all laboratory procedures and experiments are conducted in a safe and prudent manner.
5.1.26 All gas cylinders are to be properly secured and labeled.
5.1.27 All Department employees shall wear an apron, cap and slippers

(of designated colour) /shoe cover in the laboratory, as per the standard gowning procedure.
5.1.28 Analysts shall work at the respective workplace and keep them neat and clean (including table and drawers).
5.1.29 Analyst shall wear safety glasses, nose masks and hand gloves, whenever required during the testing.

5.2 Quality Control Laboratory Premise
5.2.1 Quality Control Laboratory function shall be free from dust and other disturbances.

Mopping of the floor shall be done once daily with potable grade water.
5.2.2 The premise of Quality Control department shall be well ventilated, with proper

arrangements of air conditioning. Microbiological areas have independent AHUs to avoid contamination.
5.2.3 The laboratory plan including its furniture shall be constructed in such a way to facilitate smooth working.
5.2.4 Working tables shall be smooth and resistant to most of the acids, alkalis and solvents

and easily cleanable. Floor and ceilings shall also be easily cleanable.
5.3 Instruments
5.3.1 Wherever required, instruments shall be kept in controlled temperature and humidity,

as per manufacturer recommendations.
5.3.2 All instruments shall be kept clean every time and serviced regularly as per the Annual

Maintenance Contracts and/or paid service plan with the outside agencies or from the

In-house resources. A record of the service performed on each instrument shall be maintained.
5.3.3 Instruments shall be calibrated and/or validated as per applicable SOPs. Record of

calibration and / or validation shall be maintained.
5.3.4 Standard operating procedure for each instrument shall be made available

separately for its operation and calibration.
5.3.5 All the instruments shall be switched off after use, unless required.
5.4 Glassware
5.4.1 All the glassware shall be procured from standard glassware manufacturers.
5.4.2 Glassware used for quantitative purposes shall be calibrated on the first time receipt of the

glassware and/or the frequency defined in the SOP for Calibration of Glassware.
5.4.3 Glassware shall be cleaned properly as per SOP after each analysis, and dried before next use.

The analyst shall verify the cleanliness of the glassware by visually inspection, before use.
5.4.4 After completion of analysis, glassware containing hazardous chemicals and poisonous materials

shall be emptied immediately into the sink under continuous flow of water, and then shall be kept in the washing trays.
5.5 Documentation
5.5.1 Current departmental SOPs shall be strictly followed by all employees in the laboratory.
5.5.2 Properly authorized specifications, Standard test procedure and other required work instructions

shall be available in Quality control laboratory for each analysis for each product.
5.5.3 All the materials shall be analyzed strictly as per the respective current specifications.
5.5.4 Recording of primary data shall be done in controlled protocol formats only.
5.5.5 No change in document shall be allowed unless a written authorization through proper

change control procedure is being followed.
5.5.6 After analysis all the test Protocols along with supporting raw data documents graphs,

printouts, samples, report etc. shall be submitted to Manager Quality Control/ Manager-Quality Assurance.

It shall be checked, verified and stored along with the respective document folder.
5.6 Chemicals and Reagents
5.6.1 All the chemicals shall be stored as per their properties and hazardous nature viz. inflammable,

at the designated place.
5.6.2 All the chemicals and reagents shall be labeled properly on receipt. Labels on chemicals and

reagents shall bear the standard labels.
5.6.3 All chemicals/ reference standards/ working standards shall be kept back to their respective

designated place after use and should be sealed properly.
5.6.4 Chemicals and reagents shall not be pipette through mouth. Pro-pipettes or rubber

bulbs shall be used for this purpose.
5.6.5 Chemicals and reagents taken out for analysis shall not be returned back to the

mother container. This shall be discarded.
5.6.6 Hazardous solutions shall be handled with precaution at the designated area.
5.6.7 No storage of any solutions (except stock solution with proper labels) in refrigerator or in cupboards shall be done.
5.7 Personnel Training
5.7.1 Laboratory staff shall be adequately qualified and sufficient in number to perform the assigned analytical work.
5.7.2 All the personnel shall undergo on-the job training in all Quality Control and Quality Assurance functions.
5.7.3 Each new recruit shall undergo induction training and training related to the different analysis areas.
5.8 Safety
5.8.1 Proper hand gloves, goggles and nose masks shall be used during handling of volatile corrosive chemicals.
5.8.2 First aid chart shall be available in the chemical testing room. Necessary first aid ability shall

be available in laboratory, with instant access.
5.8.3 All the electrical lines and gas lines shall be switched off after use.
5.8.4 Proper safety precautions, wherever specified, shall be followed during analysis.
5.8.5 Smoke alarms, fire extinguishers and water showers shall be installed/kept for safety purpose.
5.9 House keeping
5.9.1 Laboratory shall be neat and clean. The record of its cleaning shall be maintained as per

SOP on Good Housekeeping Practices.
5.9.2 Instruments, working tables and drawers shall be kept clean by the analyst.
5.10 Miscellaneous
5.10.1 Results of out of specifications (OOS) shall be immediately reported to

Manager- QC/ Manager-Quality Assurance. For any action anomaly, SOP on the OOS shall be followed.

6.0 Reference
In house specification

7.0 Attachments
Not Applicable

8.0 Distribution
Distribution of the controlled copy to concerned departments.
Additional copies may be issued for specific purpose.

9.0 Abbreviations
QA : Quality Assurance
QC : Quality Control
SOP : Standard Operating Procedure

10.0 Revision History