cleaning of glassware

1.0 Purpose
1.1 To describe a procedure for Cleaning of Glassware’s.

2.0 Scope
2.1 This standard operating procedure is applicable for cleaning of glassware’s in Quality Control laboratory at ABC Pvt. Ltd.

3.0 Responsibility
Officer QC/ Executive QC Preparation of SOP
Assistant Manager Reviewing and Training of SOP
Manager QC Implementation of SOP

4.0 Accountability
4.1 Manager Quality Control is accountable for implementation and compliance with SOP.

5.0 Procedure
5.1 Cleaning of General Glassware
5.1.1 After completion of Analysis, put all the used Glassware in “To Be Clean Glassware” Tray.
5.1.2 The glassware shall be cleaned immediately after completion of analysis. If the cleaning is not possible

immediately soak the glassware in water.
5.1.3 Remove the contents from the glassware carefully. The corrosive chemicals shall be first diluted in water

and then discarded in a flow of water.
5.1.4 Rinse the glassware thoroughly with tap water before cleaning glassware.
5.1.5 Dip the glassware in solution of 0.5% Labolin or 5% Extran in water for 10-15 minutes. Scrub with

nylon brush under the flow of tap water.
5.1.6 Wash the glassware with tap water till it free from foam.
5.1.7 Finally rinse the glassware with purified water.
5.1.8 After washing, glassware shall be dried in glassware drying oven at 50±5°C before use.
5.1.9 During drying glass shall be placed in oven by keeping all the glassware in an inverted position and

allowing the water to come out.
5.2 Cleaning of pipette
5.2.1 After use the pipette rinse immediately with tap water to prevent the breaking the tip not hit in sink or water tap.
5.2.2 After rinse and follow the step 5.1.5 to 5.1.9.
5.3 Cleaning of HPCL vials
5.3.1 After completion of Analysis, put out the vials from the HPLC, and drain the solution from vials in running water.
5.3.2 Rinse the vials, and septum’s with tap water.
5.3.3 Dip the vials and septum`s in solution of 0.5% Labolin or 5% Extran in 1 liter glass beaker for 30 minutes.
5.3.4 Wash the vials and septum`s with tap water till it free from foam.
5.3.5 Boil the vials in purified water for 1 hour.
5.3.6 Finally rinse the Vials and septum`s with purified water.
5.3.7 After washing Vials and septum`s shall be dried in drying oven at 50±5°C before use.
5.4 Whenever required, use Chromic acid mixture for cleaning as follows.
5.4.1 Chromic acid mixture preparation: Dissolve 200 gms of Potassium dichromate in 100 ml of

water-cooled in an ice bath. To this add very slowly 1500 ml of sulphuric acid with stirring and mix thoroughly.
Note: Chromic acid is highly corrosive wear rubber gloves, goggles during preparation.
5.4.2 Add a little quantity of chromic acid mixture in glassware and shake than wash thoroughly

with plenty of tap water and then follow from step 5.1.5 to 5.1.9.
5.4.3 New glassware shall be soaked for 1 to 2 hours in 1% v/v hydrochloric acid before washing as per step no. 5.1.5 to 5.1.9.
5.4.4 Stick stains such as grease etc. can be cleaned by 10% sodium carbonate solution or with acetone.

Never use alkalis for cleaning.
5.4.5 Allow to Cool Down the Temperature of Clean and Dried Glassware Before use

6.0 Reference
In House

7.0 Attachments
Not Applicable

8.0 Distribution
Distribution of the controlled copy to concerned departments.
Additional copies may be issued for specific purpose.

9.0 Abbreviations
QA : Quality Assurance
QC : Quality Control
SOP : Standard Operating Procedure
% : Percentage
v/v : Volume per Volume

10.0 Revision History