calibration of volumetric glassware

1.0 Purpose
1.1 To describe a procedure for Calibration of Volumetric Glassware.

2.0 Scope
2.1 This standard operating procedure is applicable for calibration of volumetric glassware in

Quality Control laboratory at ABC Pvt. Ltd., Roorkee (UK), India.

3.0 Responsibility
Officer QC/ Executive QC Preparation of SOP
Assistant Manager Reviewing and Training of SOP
Manager QC Implementation of SOP
4.0 Accountability
4.1 Manager Quality Control is accountable for implementation and compliance with SOP.

5.0 Procedure
5.1 All the volumetric glassware shall be procured from standard glassware manufacturers.
5.2 Wash the Glass Apparatus as per respective SOP.
5.3 Finally wash and Rinse the Glassware as per respective SOP.
5.4 Take the Random sample of Glassware ‘B’ class for the Calibration.
Note: For Class ‘A’ type Glassware, No calibration is required.
5.5 Calibration of Volumetric Flask:
5.5.1 Weigh the Clean and Dry Volumetric Flask / Measuring Cylinder. Let the weight be ‘X’
5.5.2 Fill the Flask with Purified Water up to the mark considering the Lower Meniscus. Weigh the Flask. Let the weight be ‘Y’.
5.5.3 Find out the weight of Purified Water by the following Formula
Weight of Purified water = Y-X = A.
5.5.4 Calculate Capacity of the Volumetric Flask by using following Formula.
– A
Capacity of Volumetric Flask (ml) =—–
-D
Where;
A = Weight of Purified Water
D = Density of Water at 25oC
5.5.5 If the Capacity of the Flask is within the Limits specified in Annexure I then accept the respective Glassware.

If the capacity of the Flask is not within the Limits specified in respective Annexure I then reject the respective Glassware.
5.6 Calibration of One Mark Volumetric Pipettes and Graduated Pipettes
5.6.1 Weigh the Clean and Dry Beaker. Let the Tare Weight be ‘X’
5.6.2 Fill the Pipette to the Lower Meniscus with Purified Water. Empty out the Purified Water in the Beaker.

Weigh the Beaker. Let the weight be ‘Y’.
5.6.3 Find out the weight of the Purified Water by the following Formula.
Weight of the Purified Water = Y-X =A
5.6.4 Calculate Capacity of the Pipette using following Formula
—-A
Capacity of pipette (ml) = —–
—D
Where;
A = Weight of Purified Water
D = Density of Water at 25oC
5.6.5 If the Capacity of the Pipette is within the limits specified in Annexure II then accept the respective

Glassware. If the Capacity of the Pipette is not within the limits specified in respective

Annexure II then reject the respective Glassware.
5.7 Calibration of Burette
5.7.1 Weigh a Clean and Dry Beaker. Let the Tare Weight be ‘X’.
5.7.2 Fill the Burette up to the Lower Meniscus of zero mark with Purified Water. Empty out

the Purified Water in the Beaker and weigh. Let the Weight be ‘Y’.
5.7.3 Find out the weight of the Purified Water by the following Formula
Weight of the Purified Water = Y-X = A
5.7.4 Calculate Capacity of the Burette using following Formula
–A
Capacity of Burette(ml) =—-
–D
Where;
A = Weight of Purified Water
D = Density of Water at 25oC
5.7.5 If the Capacity of the Burette is within the limits specified in Annexure III then accept the respective Glassware.

If the Capacity of the Burette is not within the limits specified in respective Annexure III then reject the respective Glassware
6.0 Reference
IP/ In House Specification

7.0 Attachments
Annexure: I Calibration Data Sheet for Volumetric Flasks
Annexure: II Calibration Data Sheet for Pipette.
Annexure: III Calibration Data Sheet for Burette.

8.0 Distribution
Distribution of the controlled copy to concerned departments.
Additional copies may be issued for specific purpose.

9.0 Abbreviations
QA : Quality Assurance
QC : Quality Control
SOP : Standard Operating Procedure

10.0 Revision History