General Test Procedure Identification by UV

 

1. Purpose
   1. This procedure is applicable to all incoming Raw materials, In-process and Finished products.
2. Apparatus
   1. UV-Visible spectrophotometer
   2. Quartz Cuvettes or Quartz Cells (1.0 cm)
3. Reagents
   1. Freshly distilled Water
   2. Solvents as specified : AR Grade
   3. Chemicals as specified : AR Grade
4. Procedure
   1. Set the spectrophotometer in the photometric mode. Select the specified wavelength. Prepare the test

solution as per the requirement by dissolving accurately weighed amount of sample in the specified

                      volume of solvent or diluents. Transfer the solvent or diluents, in which samples prepared, in both

                       the reference and sample cuvette.

1. 2. Place the cuvette in cuvette holders of the instrument. Auto zero the displayed reading of absorbance

                        to nullify the interference of the solvent. Remove only the sample cuvette from its holder, and rinse it

                      with about 2 to 3 ml of test solution, twice.

1. 3. Take UV absorption spectrum scan of sample and standard using 1 cm cells in the range 200 to 400

                         nm unless otherwise specified in the individual test procedure.

1. 4. The requirements are met if the Ultraviolet absorption spectrum of sample and standard exhibit

                            maxima and minima at the same wavelengths and absorptivities and or absorbance ratios are

                             within limit.

The Test solution must be clear. (Absorbance of hazy or partially dissolved substance cannot be measured.)

5. Reference
   1. IP
6. Revision History

Revision No.	Details of Changes	Reason for change
00	New GTP	NA

End of Document

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General Test Procedure Identification by UV