standard test procedure Calcium Citrate


            standard test procedure Calcium Citrate


Sr. No. TESTS Test Procedure / Methods
01. Description White odorless, crystalline powder.
02. Solubility


Freely soluble in diluted 3 N hydrochloric acid and in diluted 2 N nitric acid, slightly soluble in water, insoluble in alcohol.


        Descriptive term Parts of solvent required for part of solute
Very soluble Less than 1
Freely soluble From 1 to 10
Soluble From 10 to 30
Sparingly soluble From 30 to 100
Slightly soluble From 100 to 1000
Very slightly soluble From 1000 to 10000
Practically insoluble, or insoluble 10,000 and more
03. Identification A) Analysis: Dissolve 0.5 g in a mixture of 10 ml of water and 2.5 ml of  2N nitric acid. Add 1 ml mercuric sulfate TS, heat to boiling and add 1.0 ml of potassium permanganate. White precipitate is formed.


B) Sample: 0.5 g of calcium citrate

Analysis: ignite completely the sample at low a temperature as possible, cool and dissolve the residue in dilute glacial acetic acid (1:10). filter and add 10 ml of ammonium oxalate TS to the filtrate.

Acceptance criteria: a voluminous white precipitate that is soluble in hydrochloric acid is formed.



Arsenic Test preparation: dissolve 1 g of calcium citrate in 5 ml of 3 N hydrochloric acid, and dilute with water to 35 ml.

Acceptance criteria: NMT 3 ppm



Heavy metals Test preparation: dissolve 1 g of calcium citrate in a mixture of hydrochloride acid and water (2:20), add 1.5 ml of ammonium hydroxide and dilute with water to 25.0 ml

Acceptance criteria: NMT 20ppm

06. Lead Test preparation: Dissolve 0.5 g of calcium citrate in a 20 ml of 3 N hydrochloric acid. Evaporate this solution on a steam bath to 10 ml, dilute with water to 20 ml and cool. Use 5 ml of diluted standard lead solution (5µg of Pb) for the test. ( NMT 10 ppm)
07. Limit of fluoride (NOTE- prepare and store all solution in plastic containers)

Standard stock solution: 1000 µg/ml of fluoride ion from USP sodium fluoride WS in water.

Standard solution: 5µg/ml of  fluoride ion from standard stock solution          ( NOTE- Prepare on the day of use)

Linearity solution A: transfer 1.0 of the standard solution to a 250 ml plastic beaker. Add 50 ml of water, 5 ml of 1 N hydrochloric acid, 10 ml of 1M sodium citrate, and 10 ml of 0.2 M edetate disodium if necessary adjust with 1 N sodium hydroxide or 1 M hydrochloric acid to pH-5.5. Transfer to 100 ml volumetric flask and dilute with water to volume. This solution contains 0.05 µg/ml of fluoride.

Linearity solution B: transfer 5.0 ml of the standard solution to a 250 ml plastic beaker and proceed as directed for linearity solution A beginning with adds 50 ml of water. This solution contains 0.50 µg*/ml of fluoride.

Linearity solution C: Transfer 10.0 ml of the standard solution to a 250 ml plastic beaker and proceed as directed for linearity solution A beginning with add 50 ml of water, this solution contains 0.50µg/ml of the fluoride.

Sample solution: transfer 1.0 g of calcium citrate to a 100 ml beaker. Add 10 ml of water and, while titrating, 10 ml of 1N hydrochloric acid. When dissolved, boil rapidly for 1 min, transfer the solution to a 250 ml plastic beaker, and cool in ice water. add 15 ml of 1.0 M  sodium citrate and 10.0 ml of 0.2 M edetate disodium, and adjust with 1N sodium hydroxide or 1 M hydrochloric acid to a pH of 5.5. Transfer this solution to a100 ml volumetric flask, and dilute with water to volume.

Electrode system:   use afluoride specific, ion indicating electrode and a silver – silver chloride reference electrode connected to a pH meter capable of measuring potentials with a minimum reproducibility of ± 0.2 Mv.


Samples: linearity solution A,  linearity solution B,  linearity solution C,  and sample solution

Transfer 50 ml of each linearity solution A, linearity solution B, and linearity solution C to separate 250 ml plastic beakers, and measure the potential of each  solution with the electrode system. Between each reading wash the electrode with water and absorb any residual water by blotting the electrodes dry. plot the logarithms of the fluoride concentrations (0.05, 0.25, and 0.50µg/ml, respectively) versus potential to obtain a standard response line.

Transfer 50 ml of the sample solution to a 250 ml plastic beaker, and measure the potential with the electrode system. from the measured potential and the standard response line determine the concentration, C, in µg/ml,  of fluoride ion in the sample solution. calculate the percentage of fluoride in the specimen taken by multiplying C by 0.01.

Acceptance criteria: NMT 0.003%.

08. Limit of acid insoluble substances Sample solution: dissolve 5 g of calcium citrate by heating with a mixture of hydrochloric acid and water (10:50) for 30 minute.

Analysis: filter wash and dry at 105º for 2 h the residue so obtained.

Acceptance criteria:  The weight of the residue is NMT 10mg (2.0%).

09.0 Loss on drying Dry a sample at 150º for 4h: it loses from 10.0% to 13.3% of its weight.
10.0 Assay Procedure

Sample solution: dissolve 350 mg of calcium citrate previously dried at 150º to constant weight, in 12 ml of 0.5 M hydrochloric acid, and dilute with water to about 100 ml.

Analysis: while stirring the sample solution, add 30ml of 0.05 M disodium edetate VS from a 50 ml burette. Add 15 ml of 1 M sodium hydroxide add 300 mg of hydroxy naphthol blue, and continue the titration to a blue endpoint. each ml of 0.05 M  disodium edetate is equivalent to 8.307 mg calcium citrate.


Calculation                                             Limit 97.5 – 100.5% (on dried basis)

  Volume x 8.307 x M x 100


0.05 x wt of spl




OPA                Orthophosphoric Acid

WS                    working standard

SF                    Separating Funnel

VF                  Volumetric Flask

Std                  Standard

Spl                  Sample

wt                   weight of sample

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