Analysis method of Eberconazole Cream
1.1 To lay down a procedure for analysis of Eberconazole Cream.
2.1 This procedure is applicable to the analysis of Eberconazole Cream.in Quality control laboratory
3.1 Q.C- Chemist
4.1 Head-Quality Assurance
5.1 Description: Pour 50ml finish sample in beaker and observed visually.
5.2.1 In the assay, the principle peak in the chromatogram obtain with the test solution correspond to the peak in the chromatogram obtained with the reference solution.
5.3 Average fill weight: Weigh accurately bulk of 20 tube and calculate the average fill weight.
Average fill Weight = fill weight of 20 tube/20.
Eberconazole Nitrate IP
Eq. to Eberconazole 1.0% w/w
Estimation of Eberconazole Nitrate:
Method of Eberconazole Nitrate:
Buffer: 0.1 per cent v/v solution of Triethylamine in water adjust to pH 8.0 with orthophoshoric acid.
Mobile phase: Buffer : Methanol
25 : 75
Standard preparation: Dissolve 50mg of Eberconazole Nitrate in 100ml volumetric flask with Mobile phase. Further dilution 5.0ml to 50.0ml volumetric flask with Mobile phase.
Sample preparation: Weight accurately equivalent to 5 mg. sample of Eberconazole Nitrate. Shake with 50 ml of Mobile phase and dissolve. dilute the volume 100 ml volumetric flask with Mobile phase.
Flow rate – 1ml/mint.
column – C18(150 mm X 4.0) (5μm),
Injection volume – 20 µl.
column Temperature – 400
|Sr. No.||Abbreviation used||Full form of abbreviation used|
|1.0||STP||Standard Testing Procedure|
|Sr. No.||Reference Title|