Analysis method of Mometasone Furoate and Fusidic Acid Cream
1.1 To lay down a procedure for analysis of Mometasone Furoate and Fusidic Acid Cream.
2.1 This procedure is applicable to the analysis of Mometasone Furoate and Fusidic Acid Cream. in quality control laboratory
3.1 Q.C- Chemist
4.1 Manager-Quality Assurance
5.1 Description: Pour 50ml finish sample in beaker and observed visually.
5.2.1 In the assay, the principle peak in the chromatogram obtain with the test solution correspond to the peak in the chromatogram obtained with the reference solution.
5.3 Average fill weight: Weigh accurately bulk of 20 tube and calculate the average fill weight.
Average fill Weight = fill weight of 20 tube/20.
Mometasone Furoate I.P 0.1%
Fucidic Acid Cream 2.00% w/w
Estimation of Mometasone Furoate and Fusidic Acid:
Method of Mometasone Furoate and Fusidic Acid:
Buffer: 1.5% w/v Ammonium Acetate pH 3.8 with Orthophoshoric acid.
Mobile phase: Buffer: Acetonitrile
90 : 10
Standard preparation: Weight Accurately 20 mg. of Mometasone Furoate. And 20 mg. of Fucidic Acid add 50 ml
Mobile phase. all solution sonicates till dissolved. and dilute 100 ml volumetric flask. Further
dilution,5ml to 100ml Mometasone Furoate in100 ml of mobile phase.
Sample preparation: Weight accurately 1gm.of sample wt. Add 50 ml Mobile phase and sonicate for 20 minute. the volume
was make up 100 ml volumetric flask with Mobile phase.
Flow rate – 1.0ml/mint.
column – C8(150 mm X 3.9) (5μm),
Injection volume – 20 µl.
Column temp. – Ambient.
|Sr. No.||Abbreviation used||Full form of abbreviation used|
|1.0||STP||Standard Testing Procedure|
|Sr. No.||Reference Title|