Analysis method of Iron and Folic Acid Syrup
1.1 To lay down a procedure for analysis of Iron & Folic Acid Syrup.
2.1 This procedure is applicable to the analysis of Iron & Folic Acid Syrup QC lab
3.1 Q.C- Chemist
4.1 Manager-Quality Assurance
5.1 Description: Pour 50ml finish sample in beaker and observed visually.
5.2 pH: Taken 50 ml sample in beaker rinse the pH electrode first with purified water followed by sample dip the electrode in sample and observed the pH.
5.3 Volume variation: Measured the volume by measuring cylinder and determine the volume
5.4.1 In the assay, the principle peak in the chromatogram obtain with the test solution correspond to the peak in the chromatogram obtained with the reference solution and Chemical method.
Each 15 ml contains:
Iron (III) Hydroxide Polymaltose complex
Eq. to Element Iron 50mg.
Folic acid I.P. 0.5mg.
Estimation of Iron and Folic acid:
Method of Elemental Iron:
Method for iron:
Weigh accurately 50 mg of iron and 5 ml water. Add 3ml sulphuric acid heat and cool. Add potassium permanganate solution till Change the colour pale yellow or disappear colour add 2 gm of potassium iodide. After that 25 ml Hcl. stay on 5 to 10 minute. Add starch solution as indicator. Titrate with 0.1 M sodium Thiosulphate.
Method of Folic acid:
Method for folic acid:
Buffer: 680 mg potassium dihydrogen orthophosphate dissolve in 100 ml water. Adjust to ph-6.0 with 1N sodium hydroxide.
Mobile phase: Buffer: methanol
90 : 10
Standard preparation: weigh accurately 50 mg of folic acid in 100 ml volumetric with 0.1N sodium hydroxide. Further dilution 1.0 to 50 ml with mobile phase.
Sample preparation: weight accurately equivalent to 1.0mg of sample weight add 10 ml of 0.1 N Sodium hydroxide shake for 5 minute. And diluted to 100 ml volumetric flask.
With mobile phase.
Column c18 (250 x4.6) mm
Flow -1.2ml per minute.
Wavelength 280 nm
injection volume: 10 micro liters.
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|1.0||STP||Standard Testing Procedure|
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