Analysis method of Albendazole and ivermectin in oral liquid
1.1 To lay down a procedure for analysis of Albendazole & Ivermectin Suspension
2.1 This procedure is applicable to the analysis of Albendazole & Ivermectin Suspension in QC lab
3.1 Q.C- Chemist
4.1 Manager-Quality Assurance
5.1 Description: Pour 50ml finish sample in beaker and observed visually.
5.2 pH: Taken 50 ml sample in beaker rinse the pH electrode first with purified water followed by sample dip the electrode in sample and observed the pH.
5.3 Volume variation: Measured the volume by measuring cylinder and determine the volume variation.
5.4.1 In the assay, the principle peak in the chromatogram obtain with the test solution correspond to the peak in the chromatogram obtained obtained with the reference solution.
Each 1ml contains
Albendazole I.P. 25 mg.
Ivermectin I.P. 0.8 mg.
Method of Albendazole:
Methanolic HCL: 13 ml concentration HCL diluted to 300 ml methanol.
Standard Preparation: Taking weight 50 mg standard. of Albendazole diluted to 100 ml Methanolic Hydrochloric acid. Further dilution 1.0 ml to 50 ml with Methanolic Hydrochloric acid.
Sample preparation: weight accurately 2.15 gm sample wt. of Albendazole. diluted to 100 ml with Methanolic Hydrochloric acid. Further dilution 1.0 ml to 50 ml with Methanolic Hydrochloric acid.
Method of Ivermectin
Mobile phase: METHANOL: WATER
90 : 10
Standard preparation: Weight accurately about 40 mg standard weight of Ivermectin diluted to 100 ml volumetric flask with mobile phase. Further dilution 10 to 50 ml with methanol.
Sample preparation: Weight accurately 10 gm sample weight diluted to 100 ml volumetric flask with
Wavelength – 245 nm
Flow rate – 1.0 ml/mint
Injection – 10 µL.
Column- C18(4.6X125mm) 5 µm.
Retention time of Ivermectin – 9.0 mint.
|Sr. No.||Abbreviation used||Full form of abbreviation used|
|1.0||STP||Standard Testing Procedure|
Sr. No. Reference Title
01 In House