Analysis method of Quinine Sulphate Suspension
1.1 To lay down a procedure for analysis of Quinine Sulphate Suspension.
2.1 This procedure is applicable to the analysis of Quinine Sulphate Suspension Quality control laboratories
3.1 Q.C- Chemist
4.1 Manager-Quality Assurance
5.1 Description: Pour 50ml finish sample in beaker and observed visually.
5.2 pH: Taken 50 ml sample in beaker rinse the pH electrode first with purified water followed by sample dip the electrode in sample and observed the pH.
5.3 Volume variation: Measured the volume by measuring cylinder and determine the volume variation.
5.4.1 In the assay, the principle peak in the chromatogram obtain with the test solution correspond to the peak in the chromatogram obtained with the reference solution.
Each 5.0 ml contains:
Quinine Sulphate IP 150 mg.
Estimation of Quinine Sulphate Suspension:
Method of Quinine Sulphate Suspension:
Buffer: 0.1M Ammonium Acetate ph-7.0
Mobile phase: Buffer: Acetonitrile: Methanol
40 : 25 : 35
Standard preparation: Weight 150 mg standard weight of Quinine Sulphate diluted to 100 ml volumetric flask with Methanol.
sample preparation: Weight accurately equivalent to 150 mg. sample weight of Quinine Sulphate diluted to 100 ml volumetric flask with Methanol.
Flow rate – 1.0 ml /mint.
column -C18 (4.6mmx250) 5 micron
Temperature – Ambient Temp.
Retention time – about 7.73 mint.
Injection volume – 20µl.
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|1.0||STP||Standard Testing Procedure.|
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