Analysis method of Calcium Carbonate vitamin D3 Zinc Gluconate and Magnesium hydroxide suspension


Analysis method of Calcium Carbonate vitamin D3 Zinc Gluconate and Magnesium hydroxide suspension



1.1 To lay down a procedure for analysis of Calcium Carbonate, Vitamin D3, Zinc Gluconate and Magnesium hydroxide Suspension.


2.1 This procedure is applicable to the analysis of Calcium Carbonate, vitamin D3, Zinc Gluconate and Magnesium hydroxide Suspension quality control laboratories

3.1 Q.C- Chemist

4.1 Manager-Quality Assurance

5.1 Description: Pour 50ml finish sample in beaker and observed visually.
5.2 pH: Taken 50 ml sample in beaker rinse the pH electrode first with purified water followed by sample dip the electrode in sample and observed the pH.

5.3 Volume variation: Measured the volume by measuring cylinder and determine the volume variation.

5.4 Identification:
5.4.1 In the assay, the principle peak in the chromatogram obtain with the test solution correspond to the peak in the chromatogram obtained with the reference solution. by chemical method.
5.5 ASSAY:
Each 5.0 ml contains:
Calcium Carbonate IP (Oyster Shell) 625 mg.
Eq. to elemental calcium 250 mg.
Vitamin D3 I.P 200IU
Zinc Gluconate IP 2mg.
Magnesium hydroxide IP 75mg.

Estimation of Calcium Carbonate, Vitamin D3, Zinc Gluconate & Magnesium hydroxide
Method of Calcium Carbonate.
Weight Accurately equivalent to 100mg. sample weight dissolve in 1 ml of hydrochloric acid. add 70 ml of water, cool and add 20 ml Ammonia solution. Titrate with 0.05 M Disodium edetate 1 ml of 0.05 M Disodium edetate is equivalent to 0.00507 gm of CaCO3

V= Volume (in ml) of Disodium edetate Solution consumed in titration.

N = Actual normality of Disodium edetate Solution.

AW= Average weight.

Wt. = Weight of Sample.



Method of (vitamin D3)


Diluted solvent: Methanol

90: 10
Standard preparation: Weight accurately about 25 mg std wt. of vitamin D3 in 100 ml volumetric
Flask. Further dilution 1.0 ml to 250 ml volumetric flask with methanol.

Sample preparation: Weight accurately Equivalent to 0.1 mg of vitamin D3 and diluted to100 ml with methanol.

Chromatographic condition:

Wavelength -290nm
Flow rate – 1.0 ml /mint.
Column -c18 (150 x4.6) 5µm.
Temp – 350c
Retention time – about 5.70 mint
Injection volume – 100µl.


Method of zinc gluconate.

By titration:

Dissolve about 40 mg of Zinc gluconate, accurately weight in 100 ml of water. Add 5ml of ammonia-ammonia chloride buffer and 0.1 ml of

eriochrome blank (As indicator).To use and titrate with 0.005 M edetate disodium until the solution is deep in blue colour.

Each ml of 0.005 M edetate, disodium is equivalent to 2.278 mg of Zinc gluconate.

V= Volume (in ml) of Zinc Gluconate Solution consumed in titration.
N = Actual normality of Edetate Solution.
AW= Average weight
Wt. = Weight of Sample.





Method of Magnesium Hydroxide.
5 g of the sample is taken in 3ml of concentrated hydrochloride acid, dilute to 100ml with water, warmed on water bath and cooled.
To 25ml of sample Add 25 ml of Triethanolamine and 10 ml of Ammonia Ammonium Chloride Buffer solution. Titrate with 0.05 m E.D.T.A using erichrome black T indicator
Each ml of 0.05 M E.D.T.A is equivalent to 0.002916 of magnesium hydroxide.

Ammonia Ammonium chloride buffer:

6.75 gm Ammonium Chloride add 20 ml water and 57 ml of Ammonia produced to 100 ml water.

V= Volume (in ml) of E.D.T.A. Solution consumed in titration.

N = Actual normality of E.D.T.A Solution.

AW= Average weight.

Wt. = Weight of Sample.


Sr. No. Abbreviation used Full form of abbreviation used
1.0 STP Standard Testing Procedure
2.0 QA Quality assurance
3.0 STD Standard
4.0 SPL Sample
5.0 I.P. Indian Pharmacopeia
6.0 NM Nano meter



Sr. No.                    Reference Title
01                       In House

Bacterial endotoxin test for dexamethasone injection

Analysis of vitamin B1 B6 B2 Nicotinamide and sodium pentothenate Injection

Analysis for Nandrolone Decanoate injection

Analysis of Dicyclomine and Diclofenac sodium Injection

standard testing procedure of Fexofenadine and Phenylephrine suspension

standard testing procedure of Piroxicam Injection

STP of Fungal Diastase and Papain capsules

standard testing procedure PVC

standard testing procedure glass ampoule

Standard testing procedure of Iron Sucrose Injection

Standard testing procedure lactose

Standard testing procedure mefenamic acid

standard testing procedure domperidone

standard testing procedure flavour mixed fruit

standard testing procedure dicyclomine hydrochloride

Standard testing procedure honey pure

standard testing procedure dextromethorphan hydrobromide

standard procedure of levocarnitine injection

Analysis of Ivermectin Suspension

standard testing procedure artemether injection

standard testing procedure artemether injection

standard testing procedure Carbocisteine syrup

standard testing procedure Phytomenadione injection

standard testing procedure serratiopeptidase

standard testing procedure starch IP

standard testing procedure sucrose refined sugar

standard testing procedure titanium dioxide

standard testing procedure tramadol hydrochloride

standard testing procedure zinc sulphate

standard testing procedure croscarmellose sodium

standard testing procedure colour erythrosine supra

standard testing procedure magnesium hydroxide

standard testing procedure diclofenac sodium

standard testing procedure dibasic calcium phosphate

standard testing procedure cyanocobalamin

standard testing procedure cholecalciferol

standard testing procedure Calcium carbonate oyster shell powder

standard test procedure Calcium Citrate

standard testing procedure Bronopol

standard testing procedure Bromhexine Hydrochloride

Standard Testing Procedure diclofenac sodium injection

Standard Testing Procedure Drotaverine Hydrochloride injection

Standard Testing Procedure Tranexamic acid injection

standard test procedure paracetamol infusion

standard test procedure ofloxacin and ornidazole infusion

standard test procedure ornidazole injection

standard test procedure Ondansetron injection

standard test procedure dextrose injection

standard test procedure ciprofloxacin injection

STP and analysis method of Ammonium Chloride

Analysis method of aceclofenac

Sop for Analysis of cartons

analysis method of Losartan Potassium and Hydrochlorothiazide

analysis method of Linezolid Dry Syrup

analysis method of Drotaverine Hydrochloride and Mefenamic acid

Analysis method of Ceftriaxone Sodium and Sulbactam sodium Injection

analysis method of Cefepime and Tazobactam Injection

 Analysis method of Hydroquinone Cream

 Analysis method of Tacrolimus Ointment

 Analysis method of Terbinafine HCL Cream

 Analysis method of Mometasone Furoate and Fusidic Acid Cream

 Analysis method of Disodium Hydrogen Citrate Syrup

Analysis method of Hydroquinone with Tretinoin Cream

 Analysis method of Hydroquinone Tretinoin and Mometasone Furoate Cream

 Analysis method of Sertaconazole Nitrate Cream

 Analysis method of Halobetasol Propionate Cream

 Analysis method of Povidone Iodine with Ornidazole Ointment

Analysis method of Eberconazole Cream

 Analysis method of Luliconazole Cream

 Analysis method of Fluconazole Gel

Analysis method of Ketoconazole Cream

Analysis method of Salbutamol and Choline theophyllinate Syrup

Analysis method of Methylcobalamin Injection

Analysis method of Piroxicam and paracetamol Injection

Analysis method of Alpha Beta Arteether Injection

Analysis method of Enrofloxacin Suspension

Analysis method of Levetiracetam Syrup

Analysis method of Sucralfate suspension

Analysis method of Sucralfate and Oxetacaine Suspension

Analysis method of Quinine Sulphate Suspension

Analysis method of Calcium Carbonate vitamin D3 Zinc Gluconate and Magnesium hydroxide suspension

Analysis method of Suspension of Tribasic Calcium phosphate with vitamin D3 and Vitamin B12

Analysis method of Calcitriol with calcium citrate Suspension

Analysis method of Oxyclozanide and Fenbendazole Suspension

Analysis method of Oxyclozanide and Levamisole Suspension

Analysis method of Triclabendazole and Ivermectin Suspension

Analysis method of Itraconazole Solution

Analysis method of Levocetirizine Dihydrochloride syrup

Analysis method of Iron Calcium Vitamin D3 Folic Acid Vitamin B12 Suspension

Analysis method of Ferrous Ascorbate Cyanocobalamin and Folic Acid Suspension

Analysis method of Ambroxol Hydrochloride Drops 

Analysis method of Dried Aluminium Hydroxide Magnesium Hydroxide Oxetacaine and Simethicone suspension

Analysis method of Ferrous Ascorbate with Folic Acid suspension

Analysis method of Piracetam Syrup

Analysis method of Rafoxanide and Levamisole suspension

Analysis method of Zinc gluconate Syrup

Analysis method of Magaldrate Simethicone and Oxetacaine suspension

Analysis method of mefenamic acid and paracetamol suspension

Analysis method of Cholecalciferol Drops

Analysis method of Racecadotril suspension

Analysis method of Deflazacort Suspension

Analysis method of Montelukast sodium and levocetirizine Dihydrochloride Syrup

Analysis method of Iron and Folic Acid Syrup

Analysis method of Cyproheptadine Hydrochloride and Tricholine Citrate Syrup

Analysis method of Paracetamol Phenylephrine hydrochloride and Chlorpheniramine Maleate in oral liquid

Analysis method of Levofloxacin Hemihydrate Ornidazole and Vitamin E Solution

Analysis method of Albendazole and ivermectin in oral liquid


Leave a Comment

error: Content is protected !!