Calibration of Fourier Transform Infrared Spectrophotometer (FTIR)



Calibration of Fourier Transform Infrared Spectrophotometer (FTIR)




1.1 To lay down a procedure for the Operation and Calibration of Fourier Transform Infrared Spectrophotometer (FTIR).


2.1 This procedure is applicable for the Operation, Calibration and Maintenance of Fourier Transform Infrared Spectrophotometer (FTIR), used in the Quality Control Department

3.1 QC Officer or QC Executive

4.1 QC Manager



5.1.1 FTIR Spectrophotometry is one of the major Analytical Techniques which are being used in the Pharmaceutical Industry for the Identification

and Assay determination of various materials.
5.1.2 Fourier transform infrared (FTIR) spectroscopy is a measurement technique for collecting infrared spectra. Instead of recording

the amount of energy absorbed when the frequency of the infra-red light is varied (monochromator), the IR light is guided through an interferometer.

After passing through the sample, the measured signal is the interferogram. Performing a mathematical Fourier transform on

this signal results in a spectrum identical to that from conventional (dispersive) infrared spectroscopy.
5.1.3 FTIR spectrometers are cheaper than conventional spectrometers because building of interferometers is easier than

the fabrication of a monochromator. In addition, measurement of a single spectrum is faster for the FTIR technique because the information

at all frequencies is collected simultaneously. This allows multiple samples to be collected and averaged together resulting in an

improvement in sensitivity. Because of its various advantages, virtually all modern infrared spectrometers are FTIR instruments.
5.2 Operation
5.2.1 Before operation ensure that the instrument is clean and calibrated.
5.2.2 Turn ON the main power of the instrument and computer connected with the instrument.
5.2.3 Wait for at least 15 minutes until the initialization succeeded for proper stabilization of the instrument.
5.2.4 Before operation check the sample holder or cleanliness, if it is no clean properly it will contaminate the
Current sample and gives inaccurate results, so clean the sample die or sample holder with acetone or ethanol
and dry with tissue paper.
5.2.5 To run the software double click on the software icon on the computer desktop.
5.2.6 To open the main functioning window enter the individual user ID and password.
5.2.7 Set the general parameters as follow: Resolution : 4 cm⁻1, Measurement mode : % transmittance, No. of
Scans : 1 to 20, Scanning range : 4000 to 400 cm⁻1 or as per requirement.
5.2.8 To execute the background click on the background or reference.
5.2.9 In case of ATR (Attenuated total reflection) assembly, fix the assembly in the sample compartment, ensuring
that sample holder is empty and take air as a background scan the air without any sample in the sample

5.3 Cleaning
5.3.1 Before cleaning switch off the instrument, Clean the outer surface of the instrument with dry clean lint free
5.3.2 If required then clean the outer surface of instrument with lukewarm water and keep the surface dry.
5.3.3 Clean the sample holder with IPA or methanol and keep the sample holder and sample compartment in a dry

5.4 Safety and Precaution
5.4.1 Always keep the instrument away from direct sunlight.
5.4.2 Keep the instrument away from areas of high humidity / moisture.
5.4.3 Avoid spilling liquid into the sample compartment. Clean all spills immediately.
5.4.4 Do Not stare into the internal laser beam.
5.4.5 Do Not use a flammable gas to purge the instrument. Only use clean, dry, Oil-free Nitrogen or air to purge the instrument.
5.4.6 Do not attempt to make any adjustments, replacements or repairs to the instrument.
5.4.7 Do not operate the instrument with any cover or part removed.
5.4.8 Make sure that the sample compartment is free from dust and other foreign matter.
5.4.9 Never use the instrument when it is out of calibration.
5.4.10 Activate the silica gel bags kept in the sample compartment at 105º C for 1 hour periodically.
5.4.11 Always clean the IR assembly properly, before and after the analysis.


Sr. No.

Abbreviation used

Extended Form



Quality Control.



Standard Operating Procedure.

SOP for testing of ETP water

sop for Calibration and Maintenance of Laboratory Instruments and Equipment

Disposal of Residual Sample or Left Over Material

sop for for Laboratory Incident

standard operating procedure temperature monitoring

sop for drying of silica gel

sop for operation of infrared moisture balance

sop for preparation of mobile phase

sop for treated RO water

sop for Preparation and Issuance of Analysis protocol standard

sop of placebo and impurity stock solutions

sop for disposal of residual sample  

sop for handling of pharmacopoeial changes

sop for procedure for operation of ultrasonic cleaner

difference between UPLC and HPLC

sop for for Emergency Eyewash and Shower

sop for operation and calibration of total organic carbon analyzers

sop for operation of cobb tester

sop for Operation and calibration of atomic absorption spectrophotometer

sop for Operation and calibration of gas liquid chromatograph

sop for operation of humidity oven

sop for operation and calibration of serological water bath

sop for monitoring of drain trap

sop for destruction of analytical samples after testing and control samples

sop for destruction of used chemicals

Sop for Operation of suction pump

sop for Operation and calibration uv cabinet

sop for Operation and calibration of bulk density apparatus

sop for operation and calibration of shore hardness tester

sop for operation of rub proofness tester

sop for monitoring of purified water

sop for Retesting of packaging materials

sop for Retesting and resampling of raw materials

sop for Control of issuance of record of analysis green sheets

sop for Control of computer passwords

sop for sampling of packaging materials PM

sop for sampling of sterile raw material

sop for sampling of intermediates and finished products

sop for operation and calibration of friability test apparatus

sop for approval and rejection of packaging materials

sop for non conformance of RM PM and finished product

sop for collection storage and disposal of control samples

sop for trend analysis of finished products

sop for Chromatographic practices and system suitability

SOP For Good Laboratory Practices

sop for cleaning and operation of sieve shaker

general specification of packing material cartons

sop for Password for Analytical Instrument and LIMS software

sop for Rounding off numerical analytical results

sop for sampling of bulk and finished product

sop for cleaning of spillage material

sop for Handling of Reference Standard

sop for hplc column maintenance and washing

procedure for sampling and handling of bulk sample

STP for borewell generation point (raw water storage tank)

sop for preparation and standardization of 0.1M Zinc Sulphate

Operation &calibration of analytical balance (dhona)

Operation and Calibration Procedure for Disintegration Test apparatus

sop for preparation and standardization of 1 M Hydrochloric Acid

Preparation and standardization of 0.1 M ceric ammonium sulphate solution

sop for preparation and standardization of 0.05 m iodine solution

validation of volumetric solution 0.1m ammonium thiocyanate

handling of reference standard and preparation of working standard

sop for water sampling and analysis

sop for operation for validation of excel worksheets

sop for laboratory cleaning

sop for stability of volumetric solutions

sop for preparation of raw material in process finish product packing material data sheets

sop for handling of hazardous chemicals

sop for handling of glassware and allocation of identification number

sop for operation cleaning and calibration of bursting strength tester

sop for rounding off the analytical test results

procedure for Analyst Qualification

sop for operation and calibration of dissolution Apparatus

procedure for maintenance of desiccators

sop for for hplc column receipt checking id no and regeneration

safety data sheet for laboratory chemicals

procedure for handling of poisonous chemicals

sop for cleaning of sampling devices

sop for calibration procedure of instruments

sop for specification and standard testing raw material packing material and finished product

procedure for operation and calibration of potentiometric titrator

procedure for operating and calibration of digital hardness tester

procedure for disposal of expired chemicals, reagents and solvents

sop for behavior in quality control department

sop for preparation and standardization 0.1M sodium thiosulphate

sop for preparation and standardization 0.1M Disodium Edetate

preparation and standardization 0.1M Sodium Hydroxide Solution

Preparation and standardization of 0.1M Perchloric acid solution

sop for preparation 0.05M edetate disodium

sop for preparation 0.1M silver nitrate

sop for Operation and Calibration of High Performance Liquid Chromatography

sop for UV & Visible Spectrophotometer

procedure for Cleaning of laboratory glassware

Cleaning of Instrument, Instrument bench and surrounding area of Quality

Safety Precaution in Quality Control Department

Operation & Calibration of Analytical Balance

Calibration of Glassware in Quality Control Department

handling of samples received in Quality Control

Cleaning and Operation of Refrigerator

Operation, Cleaning and Calibration of water bath

Operation & Calibration of Refractometer

Operation and Cleaning of Centrifuge Apparatus

cleaning, operation & calibration of Vernier caliper

Calibration of Fourier Transform Infrared Spectrophotometer (FTIR)

Cleaning and operation of Moisture Analyzer

Cleaning & Operation of Vacuum pump in Quality Control Department

Operation and Calibration of Polarimeter

Cleaning and operation of Magnetic Stirrer

Cleaning Operation and Calibration of Melting Point

Operation Cleaning and Calibration of Muffle Furnance in Quality Control Department

procedure of operation and Cleaning of Sonicator

Operation Cleaning & Calibration of pH meter in Quality Control Department

Entry and Exit in Quality Control Department

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