Calibration of Fourier Transform Infrared Spectrophotometer (FTIR)
1.1 To lay down a procedure for the Operation and Calibration of Fourier Transform Infrared Spectrophotometer (FTIR).
2.1 This procedure is applicable for the Operation, Calibration and Maintenance of Fourier Transform Infrared Spectrophotometer (FTIR), used in the Quality Control Department
3.0 RESPONSIBILITY 3.1 QC Officer or QC Executive
4.0. ACCOUNTABILITY 4.1 QC Manager
5.1.1 FTIR Spectrophotometry is one of the major Analytical Techniques which are being used in the Pharmaceutical Industry for the Identification
and Assay determination of various materials. 5.1.2 Fourier transform infrared (FTIR) spectroscopy is a measurement technique for collecting infrared spectra. Instead of recording
the amount of energy absorbed when the frequency of the infra-red light is varied (monochromator), the IR light is guided through an interferometer.
After passing through the sample, the measured signal is the interferogram. Performing a mathematical Fourier transform on
this signal results in a spectrum identical to that from conventional (dispersive) infrared spectroscopy. 5.1.3 FTIR spectrometers are cheaper than conventional spectrometers because building of interferometers is easier than
the fabrication of a monochromator. In addition, measurement of a single spectrum is faster for the FTIR technique because the information
at all frequencies is collected simultaneously. This allows multiple samples to be collected and averaged together resulting in an
improvement in sensitivity. Because of its various advantages, virtually all modern infrared spectrometers are FTIR instruments. 5.2 Operation 5.2.1 Before operation ensure that the instrument is clean and calibrated. 5.2.2 Turn ON the main power of the instrument and computer connected with the instrument. 5.2.3 Wait for at least 15 minutes until the initialization succeeded for proper stabilization of the instrument. 5.2.4 Before operation check the sample holder or cleanliness, if it is no clean properly it will contaminate the Current sample and gives inaccurate results, so clean the sample die or sample holder with acetone or ethanol and dry with tissue paper. 5.2.5 To run the software double click on the software icon on the computer desktop. 5.2.6 To open the main functioning window enter the individual user ID and password. 5.2.7 Set the general parameters as follow: Resolution : 4 cm⁻1, Measurement mode : % transmittance, No. of Scans : 1 to 20, Scanning range : 4000 to 400 cm⁻1 or as per requirement. 5.2.8 To execute the background click on the background or reference. 5.2.9 In case of ATR (Attenuated total reflection) assembly, fix the assembly in the sample compartment, ensuring that sample holder is empty and take air as a background scan the air without any sample in the sample compartment.
5.3 Cleaning 5.3.1 Before cleaning switch off the instrument, Clean the outer surface of the instrument with dry clean lint free cloth. 5.3.2 If required then clean the outer surface of instrument with lukewarm water and keep the surface dry. 5.3.3 Clean the sample holder with IPA or methanol and keep the sample holder and sample compartment in a dry condition.
5.4 Safety and Precaution 5.4.1 Always keep the instrument away from direct sunlight. 5.4.2 Keep the instrument away from areas of high humidity / moisture. 5.4.3 Avoid spilling liquid into the sample compartment. Clean all spills immediately. 5.4.4 Do Not stare into the internal laser beam. 5.4.5 Do Not use a flammable gas to purge the instrument. Only use clean, dry, Oil-free Nitrogen or air to purge the instrument. 5.4.6 Do not attempt to make any adjustments, replacements or repairs to the instrument. 5.4.7 Do not operate the instrument with any cover or part removed. 5.4.8 Make sure that the sample compartment is free from dust and other foreign matter. 5.4.9 Never use the instrument when it is out of calibration. 5.4.10 Activate the silica gel bags kept in the sample compartment at 105º C for 1 hour periodically. 5.4.11 Always clean the IR assembly properly, before and after the analysis.