Operation & Calibration of Analytical Balance


Operation & Calibration of Analytical Balance


1.1 To lay down a Procedure for Operation & Calibration of Analytical Balance in Quality Control Department.


2.1 This procedure is applicable for Operation & Calibration of Analytical Balance


3.1 QC Officer or QC Executive

4.1 QC Manager



5.1 Operating procedure

5.1.1 Place weighing pan on balance, the conical peg centers the pan in the opening in the

base of the weighing chamber also place the draft cover into position.
5.1.2 The two leveling screws are adjusted so that the bubble is in the middle of the circle.
5.1.3 Connect the AC adapter to the specified power supply and plug the AC adapter’s other

terminal into the socket in the back of the balance.
5.1.4 To switch the balance on briefly touch the “On/Off” key after the main power supply of

balance has been switched on.
5.1.5 Check the level of the weighing balance; center the position of the air bubble.
5.1.6 To switch the balance off briefly touch the “On/Off” key and press it until off appears on the display.
5.1.7 Open the sliding glass door by hand.

5.1.8 Place a container or a butter paper on the weighing pan.

5.1.9 Press the →O/T ← key, the display changes to zero, Close the glass door and

then opens again when tarring is complete.

5.1.10 Place the sample to be weighed up to the desired target weight

(to read the weight accurately, the door must be closed)

5.1.11 Stability detector symbol O goes out when stability is achieved, the result is then

stable and the same is printed.

5.2 Care and Maintenance

5.2.1 To switch the balance off briefly touch the “On/Off” key and press it until off appears on the display.

5.2.2 Use a moistened soft cloth or tissue paper to clean the weighing pan and the draft cover.

5.2.3 To remove residues from the weighing chamber use the small artist’s brush.

5.2.4 Do not blow air through the chamber.

5.3 Cleaning

5.3.1 Ensure that the power supply to the instrument is switched off and main cord is removed from supply.

5.3.2 Clean the instrument with a clean dry cloth every day. A wet cloth dipped in 70 % IPA Solution may be
used occasionally. Precaution must be taken to clean the instrument immediately with dry cloth.

5.4 Precautions
5.4.1 Protect from vibrations, thermal fluctuations, sunlight, moisture & air currents.
5.5 Calibration
5.5.1 Switch ‘ON’ the power, then press gently the ‘ON/OFF’ switch on the touch screen to the switch on the balance.
5.5.2 Switch ‘ON’ the printer from the switch on the rear slide of it.
5.5.3 Press and hold the CAL/MENU switch for few seconds. The balance would do internal

calibration automatically, give out the print.
5.6 Daily Calibration of balance
5.6.1 Calibrate the balance daily with the standard weights of 1g, 10g, 100g and 200g individually.
5.6.2 When the balance stabilizes take the print out. Unload the weight and repeat the procedure for all the weights.
5.6.3 Record the observations along with the print out in the respective daily balance calibration data sheet.
5.7 Monthly Calibration of balance
5.7.1 Perform the electronic or self-calibration and check weight calibration as elaborated under “daily calibration check”.
5.7.2 In addition to the daily calibration check and perform the following calibration check.

Abbreviation Extended Form
QC Quality Control
SOP Standard Operating Procedure
mg milligram


Annexure-1 Format for Calibration (Daily)

Annexure-2 Format for Calibration (Monthly)


Format for Calibration (Monthly)


Name of the Instrument: Weighing Balance                          Instrument ID No:
Capacity: 220 gm
Date of calibration:
Next Due Date of Calibration:



Nominal wt. Actual wt. Observed wt. Limit


1. 20.0 mg 0.0200 gm ± 0.1%
2. 50.0 mg 0.0500 gm ± 0.1%
3. 100.0 mg 0.1000 gm ± 0.1%
4. 200.0 mg 0.2000 gm ± 0.1%
5. 500.0 mg 0.5000 gm ± 0.1%
6. 1.0 gm 1.0000 gm ± 0.1%
7. 10.0 gm 10.0000 gm ± 0.1%
8. 20.0 gm 19.9999 gm ± 0.1%
9. 50.0 gm 49.9999 gm ± 0.1%
10. 100.0 gm 99.9999 gm ± 0.1%
11. 200.0 gm 199.9999 gm ± 0.1%

Remarks: Satisfactory/Not satisfactory



Format for Calibration (Daily)




 Instrument ID:                                                                                                     Date:

Normal weight Actual weight Observed reading Limit Remark
50.0 mg ± 0.1%
100.0 mg ± 0.1%  


200.0 mg ± 0.1%  


500.0 mg ± 0.1%  


1.0 gm ± 0.1%  


10.0 gm ± 0.1%  


20.0 gm ± 0.1%  


50.0 gm ± 0.1%  


100.0 gm ± 0.1%
200.0 gm ± 0.1%



Result: Complies/ does not comply

Remarks: The instrument with in calibration/out of calibration limit

Done by:                                                                           Checked by:
Date:                                                                                  Date:

SOP for testing of ETP water

sop for Calibration and Maintenance of Laboratory Instruments and Equipment

Disposal of Residual Sample or Left Over Material

sop for for Laboratory Incident

standard operating procedure temperature monitoring

sop for drying of silica gel

sop for operation of infrared moisture balance

sop for preparation of mobile phase

sop for treated RO water

sop for Preparation and Issuance of Analysis protocol standard

sop of placebo and impurity stock solutions

sop for disposal of residual sample  

sop for handling of pharmacopoeial changes

sop for procedure for operation of ultrasonic cleaner

difference between UPLC and HPLC

sop for for Emergency Eyewash and Shower

sop for operation and calibration of total organic carbon analyzers

sop for operation of cobb tester

sop for Operation and calibration of atomic absorption spectrophotometer

sop for Operation and calibration of gas liquid chromatograph

sop for operation of humidity oven

sop for operation and calibration of serological water bath

sop for monitoring of drain trap

sop for destruction of analytical samples after testing and control samples

sop for destruction of used chemicals

Sop for Operation of suction pump

sop for Operation and calibration uv cabinet

sop for Operation and calibration of bulk density apparatus

sop for operation and calibration of shore hardness tester

sop for operation of rub proofness tester

sop for monitoring of purified water

sop for Retesting of packaging materials

sop for Retesting and resampling of raw materials

sop for Control of issuance of record of analysis green sheets

sop for Control of computer passwords

sop for sampling of packaging materials PM

sop for sampling of sterile raw material

sop for sampling of intermediates and finished products

sop for operation and calibration of friability test apparatus

sop for approval and rejection of packaging materials

sop for non conformance of RM PM and finished product

sop for collection storage and disposal of control samples

sop for trend analysis of finished products

sop for Chromatographic practices and system suitability

SOP For Good Laboratory Practices

sop for cleaning and operation of sieve shaker

general specification of packing material cartons

sop for Password for Analytical Instrument and LIMS software

sop for Rounding off numerical analytical results

sop for sampling of bulk and finished product

sop for cleaning of spillage material

sop for Handling of Reference Standard

sop for hplc column maintenance and washing

procedure for sampling and handling of bulk sample

STP for borewell generation point (raw water storage tank)

sop for preparation and standardization of 0.1M Zinc Sulphate

Operation &calibration of analytical balance (dhona)

Operation and Calibration Procedure for Disintegration Test apparatus

sop for preparation and standardization of 1 M Hydrochloric Acid

Preparation and standardization of 0.1 M ceric ammonium sulphate solution

sop for preparation and standardization of 0.05 m iodine solution

validation of volumetric solution 0.1m ammonium thiocyanate

handling of reference standard and preparation of working standard

sop for water sampling and analysis

sop for operation for validation of excel worksheets

sop for laboratory cleaning

sop for stability of volumetric solutions

sop for preparation of raw material in process finish product packing material data sheets

sop for handling of hazardous chemicals

sop for handling of glassware and allocation of identification number

sop for operation cleaning and calibration of bursting strength tester

sop for rounding off the analytical test results

procedure for Analyst Qualification

sop for operation and calibration of dissolution Apparatus

procedure for maintenance of desiccators

sop for for hplc column receipt checking id no and regeneration

safety data sheet for laboratory chemicals

procedure for handling of poisonous chemicals

sop for cleaning of sampling devices

sop for calibration procedure of instruments

sop for specification and standard testing raw material packing material and finished product

procedure for operation and calibration of potentiometric titrator

procedure for operating and calibration of digital hardness tester

procedure for disposal of expired chemicals, reagents and solvents

sop for behavior in quality control department

sop for preparation and standardization 0.1M sodium thiosulphate

sop for preparation and standardization 0.1M Disodium Edetate

preparation and standardization 0.1M Sodium Hydroxide Solution

Preparation and standardization of 0.1M Perchloric acid solution

sop for preparation 0.05M edetate disodium

sop for preparation 0.1M silver nitrate

sop for Operation and Calibration of High Performance Liquid Chromatography

sop for UV & Visible Spectrophotometer

procedure for Cleaning of laboratory glassware

Cleaning of Instrument, Instrument bench and surrounding area of Quality

Safety Precaution in Quality Control Department

Operation & Calibration of Analytical Balance

Calibration of Glassware in Quality Control Department

handling of samples received in Quality Control

Cleaning and Operation of Refrigerator

Operation, Cleaning and Calibration of water bath

Operation & Calibration of Refractometer

Operation and Cleaning of Centrifuge Apparatus

cleaning, operation & calibration of Vernier caliper

Calibration of Fourier Transform Infrared Spectrophotometer (FTIR)

Cleaning and operation of Moisture Analyzer

Cleaning & Operation of Vacuum pump in Quality Control Department

Operation and Calibration of Polarimeter

Cleaning and operation of Magnetic Stirrer

Cleaning Operation and Calibration of Melting Point

Operation Cleaning and Calibration of Muffle Furnance in Quality Control Department

procedure of operation and Cleaning of Sonicator

Operation Cleaning & Calibration of pH meter in Quality Control Department

Entry and Exit in Quality Control Department


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