Operation & Calibration of Refractometer

 

Operation & Calibration of Refractometer

 

 

1.0 OBJECTIVE
1.1 To lay down the Procedure for Operation & Calibration of Refractometer

2.0 SCOPE
2.1 This sop is applicable for operation & Calibration of Refractometer

3.0 RESPONSIBILITY
3.1 QC Officer or QC Executive

4.0 ACCOUNTABILITY
4.1 QC Manager

5.0 PROCEDURE


5.1 Operating Procedure
5.1.1 5.1.1 Ensure the cleanliness of Refractometer.
5.1.2 Place the instrument on a tube where adequate light can be reflected through the mirror.
5.1.3 Open the prism lock and place few drops of liquid testing sample.
5.1.4 Adjust the eye piece cross wire with a knob by rotating it in a clockwise or anti – clockwise direction

             so that the sharp demarcation line passes through its centre.
5.1.5 Sharp demarcation is produced in the form of a circle having half-light & half dark portion.
5.1.6 Fine adjustment can be done by a knob provided just above the prism..
5.1.7 Clean the instrument and keep under cover when not in use.

5.2 Calibration Procedure
To achieve accuracy the instrument should be calibrated against water, which has a refractive index of 1.3325 at 25 C against the reference liquids given below :

Reference  Liquid

Standard  R.I.at

 20 C.

Observed  R.I.

Tolerance

Carbon  Tetrachloride

1.4603

 

  0.0001

Tolerance

1.4969

 

  0.0001

O-Methylnapthalene  

1.6176

 

  0.0001

6.0 ABBREVIATIONS

Sr. No.

Abbreviation

Extended Form

02.

QC

Quality Control

03.

SOP

Standard Operating Procedure

04.

mg

milligram

05.

gm

gram


Calibration Date:


ANNEXURE I
CALIBRATION OF REFRACTOMETER


Date of Last Calibration Done:

Next Due for Calibration:

 

Reference  Liquid

Standard  R.I.at

 20 C.

Observed  R.I.

Tolerance

Carbon  Tetrachloride

1.4603

 

  0.0001

Tolerance

1.4969

 

  0.0001

O-Methylnapthalene  

1.6176

 

  0.0001

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