Operation & Calibration of Refractometer
1.0 OBJECTIVE
1.1 To lay down the Procedure for Operation & Calibration of Refractometer
2.0 SCOPE
2.1 This sop is applicable for operation & Calibration of Refractometer
3.0 RESPONSIBILITY
3.1 QC Officer or QC Executive
4.0 ACCOUNTABILITY
4.1 QC Manager
5.0 PROCEDURE
5.1 Operating Procedure
5.1.1 5.1.1 Ensure the cleanliness of Refractometer.
5.1.2 Place the instrument on a tube where adequate light can be reflected through the mirror.
5.1.3 Open the prism lock and place few drops of liquid testing sample.
5.1.4 Adjust the eye piece cross wire with a knob by rotating it in a clockwise or anti – clockwise direction
so that the sharp demarcation line passes through its centre.
5.1.5 Sharp demarcation is produced in the form of a circle having half-light & half dark portion.
5.1.6 Fine adjustment can be done by a knob provided just above the prism..
5.1.7 Clean the instrument and keep under cover when not in use.
5.2 Calibration Procedure
To achieve accuracy the instrument should be calibrated against water, which has a refractive index of 1.3325 at 25 C against the reference liquids given below :
|
Reference Liquid |
Standard R.I.at 20 C. |
Observed R.I. |
Tolerance |
|
Carbon Tetrachloride |
1.4603 |
|
0.0001 |
|
Tolerance |
1.4969 |
|
0.0001 |
|
O-Methylnapthalene |
1.6176 |
|
0.0001 |
6.0 ABBREVIATIONS
|
Sr. No. |
Abbreviation |
Extended Form |
|
02. |
QC |
Quality Control |
|
03. |
SOP |
Standard Operating Procedure |
|
04. |
mg |
milligram |
|
05. |
gm |
gram |
Calibration Date:
ANNEXURE I
CALIBRATION OF REFRACTOMETER
Date of Last Calibration Done:
Next Due for Calibration:
|
Reference Liquid |
Standard R.I.at 20 C. |
Observed R.I. |
Tolerance |
|
Carbon Tetrachloride |
1.4603 |
|
0.0001 |
|
Tolerance |
1.4969 |
|
0.0001 |
|
O-Methylnapthalene |
1.6176 |
|
0.0001 |
sop for Calibration and Maintenance of Laboratory Instruments and Equipment
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sop for for Laboratory Incident
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sop for Control of issuance of record of analysis green sheets
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sop for sampling of packaging materials PM
sop for sampling of sterile raw material
sop for sampling of intermediates and finished products
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sop for non conformance of RM PM and finished product
sop for collection storage and disposal of control samples
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