Analysis of Glucosamine 500mg Tablets

TEST PROCEDURE / METHODS

Standard testing procedure Glucosamine 500mg Tablets

01. Description: Remove 20 tablets from the strip. Place on a white paper and observe visually.

02. Average Weight: Weigh accurately 20 tablets and calculate the average weight.
Average Weight = Weight of 20 tablets / 20

03. Uniformity of weight: variation Weigh 20 tablets selected at random and determine the average weight. Weigh 20 tablets individually & record. Not more than two of the individual weights deviate from the average weight by more than 5 % and none deviates by more than 10 %.

04. Disintegration: Test Place six tablets one each in six tubes of the basket in a suitable disintegration test apparatus. Previously suspend the assembly in water (about 750-800 ml) maintained at a temperature of 37˚C ± 1˚C and operate the apparatus with disc to each tube. The tablets pass the test, if all the tablets have disintegrate (no residue of the tablet remains on the screen of the apparatus or, if a residue remains, it consists of fragments of insoluble coating of the tablets or is a soft mass with no palpable core) in 30 minutes. If 1 or 2 tablets fail to disintegrate, repeat the test on 12 additional tablets, not less than 16 of the total of 18 tablets tested disintegrate. If the tablets adhere to the disc and the preparation being examined fails to comply, repeat the test omitting the disc.

05 Leak test Equipment:
a) A vacuum Desiccator.
b) A vacuum pump with gauge.
Procedure: Take 3 strips and keep in vacuum desiccator. Ensure that strips are immersed in water. Connect the desiccator to vacuum pump with rubber tube and apply vacuum about 381 mm (15”) of mercury. The vacuum should be maintained for 30 sec. Release vacuum. Remove the strips wipe and dry with a clean cloth cut the strip and examine carefully. None of the tablets should be sticky or moist.
06 Dissolution Test Apparatus. No. 2(Paddle),
Medium. 900 ml of water
Speed and time. 75 rpm and 60 minutes.
Withdraw a suitable volume 150ml of the medium and filter, take accurately 100ml in conical flask and titrate with 0.02M AgNo3 using Potassium cromate indicator 2ml, until the solution become golden-brown.

D. Not less than 75 per cent of the stated amount of Glucosamine Sulphate Potassium Chloride.
For Glucosamine Sulphate Potassium Chloride Eq. to Glucosamine place the 296 at 500 & multiply with 0.592

07 Assay
Glucosamine Sulphate Potassium Chloride -500mg
Estimation of Glucosamine Sulphate By Titration
Procedure:- weigh Accurately glucosamine sulphate Potassium Chloride 300 mg in conical Flask, adds 100ml of water magnet for 10 minutes, further add potassium cromate indicator 2ml and titrate with 0.1N AgNO3 Volumetric Solution, until the solution become golden-brown.

(Calculate the result of labeled amount of Glucosamine Sulphate Potassium Chloride, Since the labeled amount of Glucosamine Sulphate Potassium Chloride is 500mg.
For Glucosamine Sulphate Potassium Chloride Eq. to Glucosamine multiply with 0.592.

Analysis of Glucosamine 500mg Tablets

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