standard testing procedure sucrose refined sugar

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4 years ago

standard testing procedure sucrose refined sugar

Storage Requirements:
Store protected from light and moisture.

Sampling:
Sample from each container / bag, if the consignment is of 03or less than three containers / bags. If the number of containers / bags is more than 03and are upto 100, sample at randomely using the formula root (n) +1 where n is the number of containers/bags in the consignment. Collect control sample in Pet bottle/Glass bottle.

Quantity of Composite Sample:

250 g

Description: An almost white or colourless crystal, dry crystalline powder; odourless; taste, sweet.

Solubility: Very soluble in water, freely soluble in ethanol (70%), practically insoluble in ethanol.

Identification: An orange precipitate is produced immediately.

Acidity or alkalinity: The sample solution is colourless and not more than 0.6 ml of 0.01 M sodium hydroxide is required
to change the colour of the solution to pink.

Specific optical rotation: NLT +65.90º and NMT +67.0º.

Barium: The opalescence produced by the sample solution is not more intense than that of the reference solution.

Calcium: The solution remains clear for atleast 1 minute.

Heavy Metals: NMT 10ppm.

Sulphites: A blue colour develops.

Dextrins: The sample solution remains yellow or becomes faint bluish green.

Glucose and invert sugar: The weight of the cuprous oxide is not more than 112 mg.

Colouring matter:
A. No unpleasant odour is detectable.
B. Any fluorescence is not more intense than that of a solution containing 0.4 mcg/ml of quinine sulphate in 0.005 M sulphuric acid.

Sulphated Ash: NMT 0.10% w/w.

Description: An almost white or colourless crystal, dry crystalline powder; odourless; taste, sweet.
Examine the individual samples by visually.
Reporting: Report as Complies/Does not comply.
Solubility: Use the following quantities:
Very soluble in water : 1 g in less than 1ml.
Freely soluble in ethanol (70%): 1.0 g in 1 to 10ml.
Practically insoluble in ethanol : 0.01 g in 100ml or more.
Reporting: Report as Complies/Does not comply.
Identification: Dissolve 150.0 g in sufficient carbon dioxide-free water prepared from distilled water to produce 300 ml
(solution A). Dilute 1 ml of solution A to 100 ml with water. To 5 ml of the solution add 2 ml of freshly
prepared 2 M sodium hydroxide and 0.15 ml of a freshly prepared copper sulphate solution; the solution
is clear and blue and remains so on boiling. To the hot solution add 4 ml of 2 M hydrochloric acid, heat to
boiling and add 4 ml of 2 M sodium hydroxide; an orange precipitate is produced immediately.
Reporting: Report as Complies/Does not comply.
Acidity or alkalinity: To 10 ml of solution A add 0.3 ml of phenolphthalein solution. The solution is colourless and not
more than 0.6 ml of 0.01 M sodium hydroxide is required to change the colour of the solution to
pink.
Reporting: Report as value in ml.
Specific optical rotation: Weigh accurately about 2.5 g of the sample and transfer into a 25 ml volumetric flask. Dissolve
and dilute to volume with water and mix. Determine the specific optical rotation of the
resulting solution using water as a blank at 25ºC.
Reporting: Report as value in º.
Barium: To 10 ml of solution A add 1 ml of 1 M sulphuric acid. When examined immediately and after 1 hour any
Opalescence is not more intense than that of a mixture of 1 ml of distilled water and 10 ml of solution A
Reporting: Report as Complies/Does not comply.
Calcium: To 1 ml of solution A add 9 ml of water and 1 ml of ammonium oxalate solution; the solution remains clear for
at least 1 minute.
Reporting: Report as Complies/Does not comply.
Heavy Metals: Add 0.1 ml of dilute hydrochloric acid to 4 ml of solution A and dilute with sufficient water to produce 25
ml. The solution complies with the limit test for heavy metals, Method A (10 ppm).
Reporting: Report as Complies/Does not comply.
Sulphites: To 4 ml of solution A add sufficient water to produce 20 ml, add 0.05 ml of 0.1 M iodine and 0.05 ml of starch
solution; a blue colour develops.

Reporting: Report as Complies/Does not comply.

Dextrins: To 2 ml of solution A add 8 ml of water, 0.05 ml of 2 M hydrochloric acid and 0.05 ml of 0.05 M iodine; the
solution remains yellow or becomes faint bluish green

Reporting: Report as Complies/Does not comply.

Glucose and invert sugar: Dissolve 20 g in sufficient water to make 100 ml and filter if necessary. Place 50 ml of the
clear solution in a 250-ml beaker, add 50 ml of alkaline cupric tartarate solution, cover the
beaker with a watch glass, heat the mixture at such a rate that it comes to a boil in
approximately 4 minutes and continue boiling for exactly 2 minutes. Add immediately 100 ml
of recently boiled and cooled water and collect the precipitated cuprous oxide on a tared
sintered glass crucible. Wash the residue with the hot water, then with 10 ml of ethanol (95%)
and finally with 10 ml of ther. Dry at 105º for 1 hour; the weight of the cuprous oxide is not
more than 112 mg.

Reporting: Report as a value in mg.

Colouring matter:
A. To 100 ml of solution A in a groundglass-stoppered tube add 1 ml of dilute hypophosphorous
acid and allow to stand for 1 hour; no unpleasant odour is detectable.

B. Examine solution A in ultraviolet light at 365 nm. Any fluorescence is not more intense than that
of a solution containing 0.4 µg per ml of quinine sulphate in 0.005 M sulphuric acid.

Reporting: Report as Complies/Does not comply.

Sulphated ash: Determined by dissolving 5.0 g in 5 ml of water, adding 2 ml of sulphuric acid, evaporating to dryness
and igniting to constant weight

Reporting: Report as a value in % w/w.

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