Standard testing procedure honey pure

Storage Requirements:

Store protected from light and moisture.
Sampling:
Sample from each container / bag, if the consignment is of 03or less than three containers / bags. If the number of containers / bags is more than 03and are up to 100, sample at randomly using the formula root (n) +1 where n is the number of containers/bags in the consignment. For the consignment of more than 100 containers/bags, sample additional containers/bags for every 100 containers/bags and thereafter. Collect a minimum of 5ml from each of the randomly selected containers/bags into individually no toxic, self sealing transparent polyethylene bearing ‘Sample For Analysis ‘Label kept in another transparent self sealing polythene bag. Afetr completion of sampling return rest sample on the same container. Collect control sample in Pet bottle/Glass bottle.
Quantity of Composite Sample:
1 x 100 ml

Description: Brownish clear viscous liquid.
Specific gravity: Not less than 1.25
Refractive Index: Not less than 1.4860and not more than 1.4965
Moisture content: Not more than 20.0%
Mineral content: 0.06 to 0.49g/100g.
Total water insoluble solids content: 0.01 to 0.03g/100g.

Description: Examine the individual samples by visually.
Reporting: Report as Complies/Does not comply.
Specific gravity (Relative Density): Not less than 1.017 and not more than 1.037.
Select a thoroughly clean and dry pycnometer. Calibrate the pycnometer by filling it with recently boiled and cooled water at 20º and weighing the contents. Assuming that the weight of 1 ml of water at 20º, calculate the capacity of the pycnometer. Adjust the temperature of the substance under examination, to about 20º and fill the pycnometer with it. Adjust the temperature of the filled pycnometer to 20º, remove any excess of the substance and weigh. Subtract the tare weight of the pycnometer from the filled weight of the pycnometer. Determine the weight per millilitre by dividing the weight in g, of the quantity of liquid which fills the pycnometer at the specified temperature, by the capacity expressed in ml, of the pycnometer at the same temperature.
Calculation:

Where
C is mass in g of the specific gravity pycnometer with honey sample;
A is mass in g of the empty specific gravity pycnometer.
B is mass in g of the specific gravity pycnometer with water.
Reporting: Report as value.
Refractive Index: By Refractometer

Procedure
Determine the Refractometer reading of honey at 20°C and calculate the percentage of moisture from the values given in Table1. If the determination is made at a temperature other than 20 °C, correct the reading according to the Note in Table.
Reporting: Report as value.
Moisture content: By Refractometer
Procedure: Determine the Refractometer reading of honey at 20°C and calculate the percentage of moisture from the values given in Table1. If the determination is made at a temperature other than 20 °C, correct the reading according to the Note in Table.

Table1
Refractive indices, corresponding percent soluble solids, and percent moisture in extracted honey

Refractive Index @ 20°C (Range)	Percent Soluble Solids	Percent Moisture	Refractive Index @ 20°C (Range)	Percent Soluble Solids	Percent Moisture
1.4817 – 1.4818	78.1	21.9	1.4930 – 1.4932	82.6	17.4
1.4819 – 1.4820	78.2	21.8	1.4933 – 1.4934	82.7	17.3
1.4821 – 1.4823	78.3	21.7	1.4935 – 1.4936	82.8	17.2
1.4824 – 1.4825	78.4	21.6	1.4937 – 1.4939	82.9	17.1
1.4826 – 1.4828	78.5	21.5	1.4940 – 1.4941	83.0	17.0
1.4829 – 1.4830	78.6	21.4	1.4942 – 1.4944	83.1	16.9
1.4831 – 1.4833	78.7	21.3	1.4945 – 1.4946	83.2	16.8
1.4834 – 1.4835	78.8	21.2	1.4947 – 1.4949	83.3	16.7
1.4836 – 1.4838	78.9	21.1	1.4950 – 1.4951	83.4	16.6
1.4839 – 14840	79.0	21.0	1.4952 – 1.4954	83.5	16.5
1.4841 – 1.4843	79.1	20.9	1.4955 – 1.4957	83.6	16.4
1.4844 – 1.4845	79.2	20.8	1.4958 – 1.4959	83.7	16.3
1.4846 – 1.4848	79.3	20.7	1.4960 – 1.4962	83.8	16.2
1.4849 – 1.4850	79.4	20.6	1.4963 – 1.4964	83.9	16.1
1.4851 – 1.4853	79.5	20.5	1.4965 – 1.4967	84.0	16.0
1.4854 – 1.4855	79.6	20.4	1.4968 – 1.4969	84.1	15.9
1.4856 – 1.4858	79.7	20.3	1.4970 – 1.4972	84.2	15.8
1.4859 – 1.4860	79.8	20.2	1.4973 – 1.4975	84.3	15.7
1.4861 – 1.4863	79.9	20.1	1.4976 – 1.4977	84.4	15.6
1.4864 – 1.4865	80.0	20.0	1.4978 – 1.4980	84.5	15.5
1.4866 – 1.4868	80.1	19.9	1.4981 – 1.4982	84.6	15.4
1.4869 – 1.4870	80.2	19.8	1.4983 – 1.4984	84.7	15.3
1.4871 – 1.4873	80.3	19.7	1.4985 – 1.4987	84.8	15.2
1.4874 – 1.4875	80.4	19.6	1.4988 – 1.4990	84.9	15.1
1.4876 – 1.4878	80.5	19.5	1.4991 – 1.4993	85.0	15.0
1.4879 – 1.4880	80.6	19.4	1.4994 – 1.4995	85.1	14.9
1.4881 – 1.4883	80.7	19.3	1.4996 – 1.4998	85.2	14.8
1.4884 – 1.4885	80.8	19.2	1.4999 – 1.5000	85.3	14.7
1.4886 – 1.4888	80.9	19.1	1.5001 – 1.5003	85.4	14.6
1.4889 – 1.4890	81.0	19.0	1.5004 – 1.5005	85.5	14.5
1.4891 – 1.4893	81.1	18.9	1.5006 – 1.5008	85.6	14.4
1.4894 – 1.4896	81.2	18.8	1.5009 – 1.5011	85.7	14.3
1.4897 – 1.4898	81.3	18.7	1.5012 – 1.5013	85.8	14.2
1.4899 – 1.4901	81.4	18.6	1.5014 – 1.5016	85.9	14.1
1.4902 – 1.4903	81.5	18.5	1.5017 – 1.5018	86.0	14.0
1.4904 – 1.4906	81.6	18.4	1.5019 – 1.5021	86.1	13.9
1.4907 – 1.4908	81.7	18.3	1.5022 – 1.5024	86.2	13.8
1.4909 – 1.4911	81.8	18.2	1.5025 – 1.5026	86.3	13.7
1.4912 – 1.4913	81.9	18.1	1.5027 – 1.5029	86.4	13.6
1.4914 – 1.4916	82.0	18.0	1.5030 – 1.5031	86.5	13.5
1.4917 – 1.4918	82.1	17.9	1.5032 – 1.5034	86.6	13.4
1.4919 – 1.4921	82.2	17.8	1.5035 – 1.5037	86.7	13.3
1.4922 – 1.4923	82.3	17.7	1.5038 – 1.5039	86.8	13.2
1.4924 – 1.4926	82.4	17.6	1.5040 – 1.5042	86.9	13.1
1.4927 – 1.4929	82.5	17.5	1.5043 – 1.5044	87.0	13.0
1.4912 – 1.4913	81.9	18.1	1.5027 – 1.5029	86.4	13.6
1.4914 – 1.4916	82.0	18.0	1.5030 – 1.5031	86.5	13.5
1.4917 – 1.4918	82.1	17.9	1.5032 – 1.5034	86.6	13.4
1.4919 – 1.4921	82.2	17.8	1.5035 – 1.5037	86.7	13.3
1.4922 – 1.4923	82.3	17.7	1.5038 – 1.5039	86.8	13.2
1.4924 – 1.4926	82.4	17.6	1.5040 – 1.5042	86.9	13.1
1.4927 – 1.4929	82.5	17.5	1.5043 – 1.5044	87.0	13.0

Reporting: Report as value in %.
Mineral content: By Ash
Procedure: Weight 5 g -10 g of the honey sample in an ignited and pre-weighed platinum or silica dish and gently
heat in a muffle furnace until the sample is black and dry. If necessary a few drops of olive oil may be added to prevent frothing. The sample is then ignited at 600 °C to constant weight. The sample is cooled in a desiccator and weighed.
Calculation and expression of results:

Where
M2 is the mass of the dish with the ash, g;
M is the mass of empty dish, g;
m1 is the mass of the dish with the material taken for the test, g.
The result is expressed as per cent ash (mass/mass)
Reporting: Report as value in %.
Total water insoluble solids content:
Preparation of test sample: About 20 g of honey is weighed accurately and dissolved in a suitable quantity of distilled water at 80 °C and mixed well.
Determination (gravimetric):
The test solution is filtered through a previously dried and weighed fine sintered glass crucible (pore size, 15 – 40 microns) and washed thoroughly with hot water (80°C) until free from sugars. The crucible is dried for one hour at 135 °C. Cooled and weighed to the nearest 0.1 mg.
Calculation and expression of results:
The result is expressed as per cent water insoluble solids (S), calculated as follows:

Standard testing procedure honey pure

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