Operation and calibration of BOD-Incubators

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1 month ago

Operation and calibration of BOD-Incubators

1.0 Objective
To lay down a procedure for Operation and calibration of BOD-Incubators.
2.0 Scope
This Standard Operating Procedure at Microbiology laboratory Quality control department of abc company.
3.0 Responsibility
3.1 Concerned Officer/Executive shall be responsible to follow this SOP.
3.2 Department Head or his/her designee shall be responsible to implement and ensuring to compliance of this SOP.
4.0 Abbreviations and Definitions
SOP : Standard Operating Procedure
QC : Quality Control
QA : Quality Assurance
SD : Standard deviation
5.0 Procedure
5.1 Operating instructions
5.1.1 Connect the power cable to power supply board.
5.1.2 Switch on the equipment by pressing ‘On /Off’ button of B.O.D incubator.
5.1.3 Adjust the relevant temperature by using ‘SET’ button and simultaneously rotating ‘SET’ knob

to set required temperature. Use ‘FINE’ knob for fine setting. Display will show set temperature.
5.2 Instructions to be strictly followed:
5.2.1 Keep the incubator in a room having constant temperature and away from any heating device.
5.2.2 Do not block the air passage area with samples.
5.2.3 Do not place any material on the top of equipment or do not stand on the top of the equipment.
5.3 Loading of samples into chamber:
5.3.1 Unlock the outer door and locate the place where the samples have to be place.
5.3.2 Open the inner glass door and load the samples.
5.3.3 While loading the samples following care has to be taken.
5.3.4 Sample has to stay in the confirmed area to tray.
5.3.5 Free space on the side of the trays should not be blocked by the sample.
5.3.6 Air circulation holes at the top and bottom back side of the chamber should not be blocked.
5.3.7 Sensor should not be covered with samples.
5.3.8 After completion of sample loading close the inner glass door properly and close the door latch.
5.4 Unloading of samples from chamber:
5.4.1 Unlock the outer door and locate the samples, which have to be removed.
5.4.2 Open the inner glass door and remove the samples.
5.4.3 After completion of unloading, close the inner glass door properly and close the door latch.

5.5 Cleaning of BOD Incubator:
5.5.1 Put ‘OFF’ the heating, and disconnect the power supply respectively.
5.5.2 Empty the BOD incubator by removing all the materials present inside it.
5.5.3 Clean the inner walls and trays using wet mop followed by spraying 70% Iso Propyl Alcohol and allow it to dry.
5.5.4 Ensure that the incubator is dry.
5.5.5 Replace all the materials taken from it, and switch ’ON’ the BOD incubator.
5.5.6 Frequency of cleaning shall be once in a week or during any spillage.
5.6 Calibration
5.6.1 Calibration shall be performed for Temperature accuracy in multiple locations with calibrated digital

thermometer probes by authorized out source calibration party once a year and when ever a major maintenance is done.
5.7 Acceptance criteria: Displayed temperature should be within ±2.5°C from the set temperature.
5.8 General
5.8.1 Record the displayed temperature daily at morning shift and at evening shift.
5.8.2 Displayed temperature should be within ±2.5°C from the set temperature.
5.8.3 If any abnormal observation found during the daily temperature recording, the concerned officer shall intimate to immediate supervisor immediately.
5.8.4 Document the calibration record is provided by out source calibration party.
6.0 Forms and Records
6.1 Incubator-Temperature Log – Annexure-1
7.0 Distribution
7.1 Master copy – Documentation Cell (Quality Assurance)
7.2 Control copy – Quality Control
8.0 History

Revision Number	Details For Change	Reason for Revision
00	New SOP	NA

Operation and calibration of BOD-Incubators

Reason for Revision