sop for disposal of microbiological media and cleaning of microbiological glassware
: To lay down a procedure for disposing of microbiological culture media.
Microbiologist /Q. C Executive
Quality Control Manager
4.1 Introduction :-
4.1.1 Bacteriological culture media contains all the nutritive sources which favors rapid growth of
different types of Micro-organisms. Some selective media are used for cultivating specific
type of Pathogenic organisms. The micro-organisms grown either on enriched or
selective media’s if not disposed or destroyed in proper way can cause contamination of the
environment which in turns is very hazardous.
4.2 Method :-
4.2.1 Collect all the used semi solid media’s in plastic bag containing about 50 ml of 2.5% Savlon solution and close the opening tightly with a tag or rubber-band and transfer the it to the incinerator.
4.2.2 For liquid media add 2.5% disinfectant (savlon), to the liquid media ,mix it well ,hold for half an hour and then discard them in a stream of running tap water..
4.2.3 The plates & test tubes are then thoroughly washed with soap water and sterilized by autoclaving.
5.0 Procedure for cleaning of glass-wares
Proper cleaning of glassware’s used in microbiology laboratory is very important in microbiological work. If the equipments used in microbiology are not cleaned properly, due to contamination, the microbiological results may get affected, also it can cause danger to the persons handling the equipment and risk of infection due to potential hazards of pathogenic cultures.
GMP require the standard procedure for glassware cleaning used in microbiological work. This procedure provides Guidelines for proper cleaning of glassware in micro section.
5.1) Keep all the glassware’s used in microbiology in the cupboard of the tables used by the
microbiologists to work.
5.2) Do not use these glass wares in chemical testing.
5.3) Rinse all the glass wares with tap water two to three times.
5.4) Dip all the glass wares in 2% v/v solution (prepared in D.M water)of teepol or liquid soap for at least 4 hours, for depyrogenation dip it into 2.5% NaOH solution.
5.5) Remove the glass wares from the solution.
5.6) Rinse twice with D.M water followed by rinsing once with W.F.I
5.0 ABBREVIATIONS :
W.F.I= Water For Injection, NaOH= Sodium hydroxide, %=Percentage/v=Volume by volume
D.M water= De mineralized water
4.0 REFERENCES / ANNEXURES :