sop for Media Preparation and Consumption

sop for Receipt Storage and Usage of Culture Media



1.1 To lay down a procedure for Media Preparation and Consumption.


2.1 This procedure applicable for Media Preparation and consumption In Microbiology Laboratory


3.1 Microbiologist QC


4.1 QC Manager

5.1 Requirement
5.1.1 Calibrated balance
5.1.2 Clean Spatula and Measuring cylinder
5.1.2 Non-absorbent cotton
5.1.3 Calibrated pH Meter
5.1.4 Hot Plate
5.1.5 Autoclave
5.1.6 Beaker & Conical Flask
5.1.7 Dehydrated culture media

5.2 Methodology
5.3 The dehydrated culture media as well as their ingredients are highly hygroscopic and must be stored in a cool dry place away from bright light.
5.3.1 Carefully read the instructions printed on the label of dehydrated culture media containers. Check the composition of the medium and the direction for its use.
5.3.2 Examine the physical nature of the dehydrated medium. If any unusual color, odor or physical appearance is noticed, discard the medium.
5.3.3 Always use a dry spoon or spatula for weighing the dehydrated media. The weighing operation shall be completed as quickly

as possible to prevent absorption of moisture by the hygroscopic contents. Wear hand gloves & nose mask while weighing the

dehydrated media to avoid inhalation of fine particles of media.
5.3.4 Operate the balance and weigh accurately the required quantity of dehydrated culture medium on a butter paper transfer the powder to a suitable container.
5.3.5 Add a part of the required quantity of Purified Water and mix the contents. Make up the volume with Purified Water & mix well.
5.3.6 Check the pH of media at 25ºC±2ºC. If there is requirement of pH adjustment, adjust the pH with 1M NaOH or 1M HCl.
5.3.7 Now heat the contents with frequent agitation till the contents are homogenous.
5.3.8 Dispense the reconstituted medium in suitable containers like test tubes, bottles or flasks. The volume of these containers shall be sufficiently

large to facilitate proper aeration and avoid overflow of medium during sterilization.
5.3.9 All culture media containers shall be plugged with non absorbent cotton and covered with a butter paper/aluminum foil.
5.3.10 All the media tubes and flasks should be labeled with details like Name of Media, Media Lot No., Date of Preparation.
5.3.11 Media after preparation and dispensing as above shall be sterilized as soon as possible. Media prepared and dispensed as

above shall be sterilized in an autoclave at 121°C/115°C for 15 minutes.
5.3.12 Specified limits as directed on label of media bottle, discard the media as per SOP for destruction of microbial waste and contaminated media by autoclave SOP.
5.3.13 If the medium is not used in the same day of preparation, it should be stored properly for not more than 7 days.
5.3.14 Stored Media showing any change in appearance or any sign of visible microbial contamination or cracks or dehydration shall be discarded.
5.3.15 Enter details of prepared media in the ‘Media Preparation & Consumption Record’



Abbreviation used Full form of abbreviation used
SOP Standard Operating Procedure
IPA Iso Propyl Alcohol
QA Quality assurance
SCDM Soyabean Casein Digest Medium
IP Indian Pharmacopeia

sop for calibration and validation of micro autoclave

sop for Sterility failure investigation

cleaning and operation of discard autoclave

sop for operation of fogger machine

sop for Biological assay of lactic acid bacillus

sop for preparation of culture inoculum

STP for sterility testing of sterile gloves

sop for Operation and calibration of active air sampler

sop for transfer of material for testing and sampling in sterile area

entry & exit procedure in microbiology laboratory

Growth Promotion Test In Microbiology Laboratory

Operation of B.O.D in Microbiology Laboratory

Operation of Horizontal Laminar Air Flow in the microbiology laboratory

Operation and cleaning of Pass Box.

Operation and cleaning of air sampler

Cleaning and Sterilization of Glassware

Analysis of water for microbial load in microbiology lab

Operation and temperature monitoring of Refrigerator

Fumigation of Microbiology Laboratory.

Entry & Exit procedure In Sterility Area

SOP for Microbial analysis of Raw Material Finished Products

SOP for Operation & Calibration of pH meter in Micro Department

SOP for Operation & Calibration of pH meter in Micro Department

SOP Operation and calibration of Hot Air Oven In Microbiology

SOP for operation cleaning & calibration of Digital colony counter

SOP for Operation And Cleaning of Microscope

sop for Media Preparation and Consumption

sop for Receipt Storage and Usage of Culture Media

sop for Cleaning Sanitization And Disinfection In Microbiology

sop for Environmental monitoring of all the Classified area

sop for Handling and Sub culturing of Microbial cultures

sop for Media Growth Promotion Test and various Microbiological test

sop for BOD incubator operation and cleaning

sop sampling of water for microbiological analysis

sop for Disinfectant Efficacy Test

sop for for cleaning and operation of vortex mixture

sop for Temperature & Relative Humidity Monitoring

sop for Operation and Calibration of Heating Block

sop for Sterility Testing of Microbiology

sop for Disposal of Culture Media

sop for Drain point of Microbiology

sop for entry & exit procedure In Microbial limit test and Biosafety

sop for Gram Staining of Bacteria in Microbiology Laboratory

sop for Monitoring of Compressed Air/gases for microbiological analysis

sop for BET (Bacterial Endotoxin) test in Microbiology

sop for receipt storage and Determining the population of Biological indicators

sop for qualification of analyst microbiologist

sop for Bioburden test of Packing materials in Microbiology Laboratory

sop for microbiological assay of erythromycin antibiotic

sop for liquid particle counter

sop for operation and calibration of digital zone reader

sop for monitoring of ultraviolet efficiency LAF and pass box

microbiological assay of cyanocobalamin or vitamin B12

gowning procedure for microbiological testing area

swab testing of various surfaces for bioburden determination

sop for endotoxin challenge test

Hold time study protocol for sterilized media

sop for personnel Qualification protocol for aseptic area

sop for sampling and testing of drain water

Sop for Operation of Airborne Particle Counter

sop for Inoculum Preparation

sop for Validation protocol of steam sterilizer autoclave

sop for pathogen detection from drain point

Sop for Analysis of Raw water Purified water water for injection and pure steam water

sop for preservatives efficacy test

sop for collection and preservation of in house isolated microorganisms

sop for Operation Calibration and Maintenance of Micropipette

Sop for UV Efficacy Test

sop for gram staining

Sop for swab testing

sop for microbiological testing of water


sop for depyrogenation of apparatus

sop for media preparation

sop for fertility test growth promotion test of media

sop for Operation and cleaning of moist heat sterilizer

sop for monitoring by active air sampler

sop for swab sampling and testing for clean rooms in production area

sop for monitoring in microbiology laboratory

sop for Fumigation of aseptic area and microbiology lab

sop for monitoring of personnel in aseptic area

sop for maintenance of cultures

sop for Operation and cleaning of laminar bench

preparation of settle plates

sop for monitoring of pure steam

sop for entry and exit procedure to m.l.t and b.e.t room

sop for storage of and use of media

sop for disposal of microbiological media and cleaning of microbiological glassware

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