Operation and cleaning of air sampler
1.1 To lay down the Procedure for operation and cleaning of air sampler.
2.1 This procedure applicable for operation and cleaning of air sampler of Microbiology Laboratory
3.1 Microbiologist QC
4.1 QC Manager
5.1.1 Sterile SCDA plates
5.1.2 70% IPA solution
5.2.1 Prepare SCDA medium as per SOP for preparation of culture media.
5.2.2 Aseptically pour approximately 15 – 20 ml of sterile molten cooled (45°C) SCDA agar into sterile 90 mm petri plates.
5.2.3 Allow to solidify the plates under LAF, after solidification label all the plates with name of media, and date of preparation.
5.2.4 Invert and incubate the plates at 30 to 35°C for 24 to 48 hours. After incubation check the plates for any contamination,
if there is contamination discard the plates as per SOP for Destruction of microbial waste and expired media by autoclaving.
5.2.5 After pre-incubation, label all the plates with date of sampling and location with the help of marker pen and wrap with aluminum foil.
5.2.6 Autoclave the SS head of Air Sampler at 121°C for 30 minutes. Transfer the container and Air sampler which is sanitized and
wrapped in aluminum foil, to sterile area through Dynamic pass box of the respective sterile area and personnel must
be entered through Change room as per SOP for Entry & Exit procedure.
5.2.7 Place the pre incubated SCDA plates in Air sampler holder and operate the air sampler for 10 minutes.
5.2.8 After 10 minutes of Air sampling, remove the plates from Air sampler, close the lid immediately and place aside.
5.2.9 Immediately clean the Air sampler with 70% sterile IPA solution and carry out the air sampling for other specified locations.
5.2.10 After air sampling collect all the plates and send to Incubator room for incubation.
5.2.11 Incubate one plate as negative control.
5.2.12 Invert all the plates and incubate at 20 to 25°C for 72 hrs for fungi and then 30 to 35°C for 48 hrs for bacteria.
5.2.13 After incubation, count the number of colony forming units with the help of colony counter. Operate the colony
counter as per SOP and express the result cfu/m3.
5.2.14 Record the result as per the following formula :
X= Pr X 1000/V
X = cfu/m3
r = colony forming units counted on 90 mm plates
Pr = Probable count obtained by positive hole correction from table I against r value
V = Volume of air sampled
|Full form of abbreviation used
|Standard Operating Procedure
|Iso Propyl Alcohol