sop for entry & exit procedure In Microbial limit test and Biosafety
1.0 OBJECTIVE
1.1 To lay down a procedure for entry & exit procedure in MLT and Biosafety.
2.0 SCOPE
2.1 This procedure applicable to entry & exit procedure In MLT and Biosafety
3.0 RESPONSIBILITY
3.1 Microbiologist QC
4.0 ACCOUNTABILITY
4.1 QC Manager
5.0 PROCEDURE
5.1 Entry Procedure
5.2 First change room
5.2.1 Fill Entry/Exit log book and then remove accessories and slippers .
5.2.2 Disinfectant the hand with filtered 70% IPA.
5.2.3 Push the doors of the change room with elbows.
5.3 Second change room
5.3.1 Disinfectant the hand through. 70% IPA.
5.3.2 Wear the sterilized surgical gloves taking care not to contaminate the outer surface of gloves With hands.
5.3.3 Switch OFF the UV light of Garment Cubicle and then open its door.
5.3.4 Take out a set of sterilized garment.
5.3.5 Sanitize the hand with 70% IPA , pull the door and enter MLT and Biosafety area.
5.3.6 Wear the sterilized garments in the following sequence.
5.3.7 Wear the hood, ensuring that the hair is properly tucked inside the hood.
5.3.8 Wear the sterile suit. Check gowning by standing in front of mirror.
5.3.9 Wear & tie the booties.
5.3.10 Disinfect the hands with 70% IPA.
5.3.11Enter in to MLT and Biosafety room by opening door with elbow.
5.4 Precaution
5.5.1 Do not put the sterile dress on the floor while changing, as this could contaminate outer surface of the dress.
5.5.2 Do not allow other person to enter.
5.5.3 Strictly follow the Entry and Exit procedure all the times.
5.5.4 Do not open two door of the Air lock at the same time
5.4.5 Open the doors of change rooms with elbows.
5.4.6 Wear only sterile dress that are kept in garment cubicle.
5.4.7 Sterile dresses kept in garment cubicle should be wrapped in butter paper.
6.0 ABBREVIATIONS
Abbreviation used | Extended Form |
MLT | Microbial limit test |
QA | Quality Assurance |
CH | Change Room |
QC | Quality Control |
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sop for entry & exit procedure In Microbial limit test and Biosafety
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sop for pathogen detection from drain point
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sop for Operation Calibration and Maintenance of Micropipette
sop for microbiological testing of water
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sop for entry and exit procedure to m.l.t and b.e.t room
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