sop for BOD incubator operation and cleaning

sop for BOD incubator operation and cleaning


1.1 To lay down the procedure for Operation Of B.O.D in Microbiology Laboratory.

2.1 This procedure applicable for Operation Of B.O.D in Microbiology Laboratory BOD Incubator (Bio-Oxygen Demand) are used to maintain temperature for test tissue culture growth, storage of bacterial cultures and incubation where high degree of constant temperature accuracy is required.

3.1 Microbiologist QC

4.1 Q. C. Manager

5.1 Operation
5.1.0 Remove the cover of microscope and clean the lens with lens paper or lint free duster before using it. Remove oily substance from

glass parts by wiping with a lint free duster or lens paper moistened with xylene.
5.1.1 Remove the xylene immediately with lens paper dipped with 95% alcohol and then wipe the lens with fresh lens paper.
5.1.2 Connect the 230V AC power supply to the power connection properly and Switch “ON” the Main.
5.1.3 Halogen bulb glows as the power switch is operated in ON position.

5.1.4 Prepare a thin smear on specimen slide and place on the platform of microscope.

5.1.5 Rotate the revolving nosepiece to engage the desired objective in the light path i.e. 10X, 40X or 100X.

5.1.6 Look at the image through the eyepiece with your eye and focus on the specimen with the focus adjustment knobs.

5.1.7 When the image comes into sharp focus with low power objective, rotate the nose piece to the next

lens (i.e. 40X or 100X) by simply swing the objective into desired place.

5.1.8 When using oil-immersion objective, swing the high power objective partially out of way and place

a drop of immersion oil on the area of slide you are observing and bring the oil-immersion objective into position.
5.1.9 Now look through the eye piece and focus the object with fine adjustment knob.
5.1.10 Before putting cover of microscope, after completion of work, move the nose piece to bring low power objective into position,

clean the oil from the immersion lens and also clean off side with tissue paper.
Cleaning : Daily
5. Wipe the outer surface of the instrument with Colin solution.
Keep the microscope and its parts clean and handle all parts with care.
Glass parts should not be touched with finger.
Always keep the microscope covered when not in use.
Never use a slide without cover slip while examining an object under high power of the microscope; otherwise the chemicals will damage the objective.

Abbreviation used Full form of abbreviation used
SOP Standard Operating Procedure
QC Quality Control
MB Microbiology Lab
BOD Bio-Oxygen demand
°C Degree Centigrade

sop for calibration and validation of micro autoclave

sop for Sterility failure investigation

cleaning and operation of discard autoclave

sop for operation of fogger machine

sop for Biological assay of lactic acid bacillus

sop for preparation of culture inoculum

STP for sterility testing of sterile gloves

sop for Operation and calibration of active air sampler

sop for transfer of material for testing and sampling in sterile area

entry & exit procedure in microbiology laboratory

Growth Promotion Test In Microbiology Laboratory

Operation of B.O.D in Microbiology Laboratory

Operation of Horizontal Laminar Air Flow in the microbiology laboratory

Operation and cleaning of Pass Box.

Operation and cleaning of air sampler

Cleaning and Sterilization of Glassware

Analysis of water for microbial load in microbiology lab

Operation and temperature monitoring of Refrigerator

Fumigation of Microbiology Laboratory.

Entry & Exit procedure In Sterility Area

SOP for Microbial analysis of Raw Material Finished Products

SOP for Operation & Calibration of pH meter in Micro Department

SOP for Operation & Calibration of pH meter in Micro Department

SOP Operation and calibration of Hot Air Oven In Microbiology

SOP for operation cleaning & calibration of Digital colony counter

SOP for Operation And Cleaning of Microscope

sop for Media Preparation and Consumption

sop for Receipt Storage and Usage of Culture Media

sop for Cleaning Sanitization And Disinfection In Microbiology

sop for Environmental monitoring of all the Classified area

sop for Handling and Sub culturing of Microbial cultures

sop for Media Growth Promotion Test and various Microbiological test

sop for BOD incubator operation and cleaning

sop sampling of water for microbiological analysis

sop for Disinfectant Efficacy Test

sop for for cleaning and operation of vortex mixture

sop for Temperature & Relative Humidity Monitoring

sop for Operation and Calibration of Heating Block

sop for Sterility Testing of Microbiology

sop for Disposal of Culture Media

sop for Drain point of Microbiology

sop for entry & exit procedure In Microbial limit test and Biosafety

sop for Gram Staining of Bacteria in Microbiology Laboratory

sop for Monitoring of Compressed Air/gases for microbiological analysis

sop for BET (Bacterial Endotoxin) test in Microbiology

sop for receipt storage and Determining the population of Biological indicators

sop for qualification of analyst microbiologist

sop for Bioburden test of Packing materials in Microbiology Laboratory

sop for microbiological assay of erythromycin antibiotic

sop for liquid particle counter

sop for operation and calibration of digital zone reader

sop for monitoring of ultraviolet efficiency LAF and pass box

microbiological assay of cyanocobalamin or vitamin B12

gowning procedure for microbiological testing area

swab testing of various surfaces for bioburden determination

sop for endotoxin challenge test

Hold time study protocol for sterilized media

sop for personnel Qualification protocol for aseptic area

sop for sampling and testing of drain water

Sop for Operation of Airborne Particle Counter

sop for Inoculum Preparation

sop for Validation protocol of steam sterilizer autoclave

sop for pathogen detection from drain point

Sop for Analysis of Raw water Purified water water for injection and pure steam water

sop for preservatives efficacy test

sop for collection and preservation of in house isolated microorganisms

sop for Operation Calibration and Maintenance of Micropipette

Sop for UV Efficacy Test

sop for gram staining

Sop for swab testing

sop for microbiological testing of water


sop for depyrogenation of apparatus

sop for media preparation

sop for fertility test growth promotion test of media

sop for Operation and cleaning of moist heat sterilizer

sop for monitoring by active air sampler

sop for swab sampling and testing for clean rooms in production area

sop for monitoring in microbiology laboratory

sop for Fumigation of aseptic area and microbiology lab

sop for monitoring of personnel in aseptic area

sop for maintenance of cultures

sop for Operation and cleaning of laminar bench

preparation of settle plates

sop for monitoring of pure steam

sop for entry and exit procedure to m.l.t and b.e.t room

sop for storage of and use of media

sop for disposal of microbiological media and cleaning of microbiological glassware

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