sop for BOD incubator operation and cleaning
1.0 OBJECTIVE
1.1 To lay down the procedure for Operation Of B.O.D in Microbiology Laboratory.
2.0 SCOPE
2.1 This procedure applicable for Operation Of B.O.D in Microbiology Laboratory BOD Incubator (Bio-Oxygen Demand) are used to maintain temperature for test tissue culture growth, storage of bacterial cultures and incubation where high degree of constant temperature accuracy is required.
3.0 RESPONSIBILITY
3.1 Microbiologist QC
4.0 ACCOUNTABILITY
4.1 Q. C. Manager
5.0 PROCEDURE
5.1 Operation
5.1.0 Remove the cover of microscope and clean the lens with lens paper or lint free duster before using it. Remove oily substance from
glass parts by wiping with a lint free duster or lens paper moistened with xylene.
5.1.1 Remove the xylene immediately with lens paper dipped with 95% alcohol and then wipe the lens with fresh lens paper.
5.1.2 Connect the 230V AC power supply to the power connection properly and Switch “ON” the Main.
5.1.3 Halogen bulb glows as the power switch is operated in ON position.
5.1.4 Prepare a thin smear on specimen slide and place on the platform of microscope.
5.1.5 Rotate the revolving nosepiece to engage the desired objective in the light path i.e. 10X, 40X or 100X.
5.1.6 Look at the image through the eyepiece with your eye and focus on the specimen with the focus adjustment knobs.
5.1.7 When the image comes into sharp focus with low power objective, rotate the nose piece to the next
lens (i.e. 40X or 100X) by simply swing the objective into desired place.
5.1.8 When using oil-immersion objective, swing the high power objective partially out of way and place
a drop of immersion oil on the area of slide you are observing and bring the oil-immersion objective into position.
5.1.9 Now look through the eye piece and focus the object with fine adjustment knob.
5.1.10 Before putting cover of microscope, after completion of work, move the nose piece to bring low power objective into position,
clean the oil from the immersion lens and also clean off side with tissue paper.
Cleaning : Daily
5. Wipe the outer surface of the instrument with Colin solution.
Precaution
Keep the microscope and its parts clean and handle all parts with care.
Glass parts should not be touched with finger.
Always keep the microscope covered when not in use.
Never use a slide without cover slip while examining an object under high power of the microscope; otherwise the chemicals will damage the objective.
6.0 ABBREVIATIONS
| Abbreviation used | Full form of abbreviation used |
| SOP | Standard Operating Procedure |
| QC | Quality Control |
| MB | Microbiology Lab |
| BOD | Bio-Oxygen demand |
| °C | Degree Centigrade |
sop for calibration and validation of micro autoclave
sop for Sterility failure investigation
cleaning and operation of discard autoclave
sop for operation of fogger machine
sop for Biological assay of lactic acid bacillus
sop for preparation of culture inoculum
STP for sterility testing of sterile gloves
sop for Operation and calibration of active air sampler
sop for transfer of material for testing and sampling in sterile area
entry & exit procedure in microbiology laboratory
Growth Promotion Test In Microbiology Laboratory
Operation of B.O.D in Microbiology Laboratory
Operation of Horizontal Laminar Air Flow in the microbiology laboratory
Operation and cleaning of Pass Box.
Operation and cleaning of air sampler
Cleaning and Sterilization of Glassware
Analysis of water for microbial load in microbiology lab
Operation and temperature monitoring of Refrigerator
Fumigation of Microbiology Laboratory.
Entry & Exit procedure In Sterility Area
SOP for Microbial analysis of Raw Material Finished Products
SOP for Operation & Calibration of pH meter in Micro Department
SOP for Operation & Calibration of pH meter in Micro Department
SOP Operation and calibration of Hot Air Oven In Microbiology
SOP for operation cleaning & calibration of Digital colony counter
SOP for Operation And Cleaning of Microscope
sop for Media Preparation and Consumption
sop for Receipt Storage and Usage of Culture Media
sop for Cleaning Sanitization And Disinfection In Microbiology
sop for Environmental monitoring of all the Classified area
sop for Handling and Sub culturing of Microbial cultures
sop for Media Growth Promotion Test and various Microbiological test
sop for BOD incubator operation and cleaning
sop sampling of water for microbiological analysis
sop for Disinfectant Efficacy Test
sop for for cleaning and operation of vortex mixture
sop for Temperature & Relative Humidity Monitoring
sop for Operation and Calibration of Heating Block
sop for Sterility Testing of Microbiology
sop for Disposal of Culture Media
sop for Drain point of Microbiology
sop for entry & exit procedure In Microbial limit test and Biosafety
sop for Gram Staining of Bacteria in Microbiology Laboratory
sop for Monitoring of Compressed Air/gases for microbiological analysis
sop for BET (Bacterial Endotoxin) test in Microbiology
sop for receipt storage and Determining the population of Biological indicators
sop for qualification of analyst microbiologist
sop for Bioburden test of Packing materials in Microbiology Laboratory
sop for microbiological assay of erythromycin antibiotic
sop for liquid particle counter
sop for operation and calibration of digital zone reader
sop for monitoring of ultraviolet efficiency LAF and pass box
microbiological assay of cyanocobalamin or vitamin B12
gowning procedure for microbiological testing area
swab testing of various surfaces for bioburden determination
sop for endotoxin challenge test
Hold time study protocol for sterilized media
sop for personnel Qualification protocol for aseptic area
sop for sampling and testing of drain water
Sop for Operation of Airborne Particle Counter
sop for Validation protocol of steam sterilizer autoclave
sop for pathogen detection from drain point
Sop for Analysis of Raw water Purified water water for injection and pure steam water
sop for preservatives efficacy test
sop for collection and preservation of in house isolated microorganisms
sop for Operation Calibration and Maintenance of Micropipette
sop for microbiological testing of water
sop for depyrogenation of apparatus
sop for fertility test growth promotion test of media
sop for Operation and cleaning of moist heat sterilizer
sop for monitoring by active air sampler
sop for swab sampling and testing for clean rooms in production area
sop for monitoring in microbiology laboratory
sop for Fumigation of aseptic area and microbiology lab
sop for monitoring of personnel in aseptic area
sop for maintenance of cultures
sop for Operation and cleaning of laminar bench
sop for monitoring of pure steam
sop for entry and exit procedure to m.l.t and b.e.t room
sop for storage of and use of media
sop for disposal of microbiological media and cleaning of microbiological glassware