sop sampling of water for microbiological analysis

sop sampling of water for microbiological analysis



1.1 To lay down the procedure for the sampling of Water for Microbiological Analysis.


2.1 This procedure applicable for the sampling of Water for Microbiological Analysis In Microbiology Laboratory


3.1 Microbiologist QC


4.1 QC Manager


5.1 Requirement
5.1.1 Sampling bottles
5.1.2 70% IPA.
5.1 Methodology
5.2.1 Before sampling ensure that the all sampling requirement available as mentioned..

5.2.2 Refer sampling points for a particular day.
5.2.3 Sample for microbiological analysis should be collected in pre-sterilized screw cap bottles.
5.2.4 Wear the sterilized hand gloves and remove the cap of bottle just prior collecting the sample.
5.2.5 The ‘sample contact area of the cap, should not come in contact with any surface, including the

fingers or hands of the individual collecting the sample.
5.2.6 Open the sampling/user point valve until there is steady stream of water.
5.2.7 Drain for approximately 1 minute, collect the water in sampling bottle.
5.2.8 Do not allow the bottle, or ‘water of bottle, come in contact with the valve.
5.2.9 Fill the bottle without overflowing the bottle.
5.2.10 Remove the bottle from the sample stream and place the cap on the bottle as quickly as possible.
5.2.11Tighten the cap securely.
5.2.12 Close the valve.
5.2.13 Fill the information on the face of the bottle such as location of sampling, Date of sampling.
5.2.14Collect all the sample and proceed for the analysis as per the SOP of water analysis (FPPL/MB/008-00).

If the sample are not taken immediately for analysis it store it at refrigerator 2-8 oC.
5.2.15Ensure that analysis is initiated in between within 2hrs after sampling.


Abbreviation used Extended Form
SOP Standard Operating Procedure
IPA Iso Propyl Alcohol
QA Quality Assurance
oC Degree Centigrade
% Percentage

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STP for sterility testing of sterile gloves

sop for Operation and calibration of active air sampler

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sop for pathogen detection from drain point

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sop for preservatives efficacy test

sop for collection and preservation of in house isolated microorganisms

sop for Operation Calibration and Maintenance of Micropipette

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Sop for swab testing

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sop for entry and exit procedure to m.l.t and b.e.t room

sop for storage of and use of media

sop for disposal of microbiological media and cleaning of microbiological glassware

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