sop for operation and calibration of digital zone reader

To describes the procedure for Operation and Calibration of Digital Zone reader.
It is the policy of Analytical Division that a written procedure shall be followed for the Operation and Calibration of Digital Zone reader.
This SOP shall be applicable for Digital Zone reader in Microbiology Laboratory of Analytical Division
Persons along with their responsibilities are given below:
S. No Designation Responsibility
01 Microbiologist Preparation & revision of SOP.
02 Officer- QA Distribution and retrieval of SOP.
03 Technical manager Training of SOP.
04 Quality Manager Approval & Implementation of SOP.


5.1.1 Ensure the cleanliness of Digital Zone reader. All exposed parts including lens, zone glass display should be clean and dry.
5.1.2 Switch ‘ON’ the main power supply.
5.1.3 There is a ‘ON / OFF’ switch on the front side of the instrument. Put this switch to ‘ON’ position.
5.1.4 The indicator lamp shall be ‘ON’ and the main light source of the Digital Zone reader shall be ‘ON’.
5.1.5 Place the Microbiological assay plate and measure accurately the zone.
5.1.6 Keep the Petri-dish on the movable Platform. Rotate the Petri dish manually, in a manner so that one end of the Zone image just touches the mark line on the Zone glass display.
5.1.7 Press the ZERO set button and display to zero.
5.1.8 Keep the plate on the stage. Adjust the plate such that one side of circle of the zone touches the marked line of the Zone glass display.
5.1.9 Turn the knob in clockwise direction so that the plate moves across the marked line of the Zone glass display until the opposite edge of the zone is in tangent to the marked line of the Zone glass display.
5.1.10 Note down the measurement of zone by the help of display reading.
5.1.11 Record the ON and OFF time of Digital Zone reader in the Format No. MPL/QM/SOP-56/FR-01/Rev-00.
5.1.12 Switch ‘OFF’ the mains and keep the instrument when not in use.
Frequency: Yearly for External calibration.
Proper handling of instrument.
Instrument should be clean and free from dust.
Zone glass display shall be wiped / cleaned with clean and dry tissue paper.
If the instrument does not provide a require light or its response is poor then it should be labeled out of order and should we repair or serviced.

Log book format for Digital Zone


QA Quality Assurance
SOP Standard Operating Procedure
QM Quality Microbiology
FR Format
S. No. Serial Number

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Cleaning and Sterilization of Glassware

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sop for Environmental monitoring of all the Classified area

sop for Handling and Sub culturing of Microbial cultures

sop for Media Growth Promotion Test and various Microbiological test

sop for BOD incubator operation and cleaning

sop sampling of water for microbiological analysis

sop for Disinfectant Efficacy Test

sop for for cleaning and operation of vortex mixture

sop for Temperature & Relative Humidity Monitoring

sop for Operation and Calibration of Heating Block

sop for Sterility Testing of Microbiology

sop for Disposal of Culture Media

sop for Drain point of Microbiology

sop for entry & exit procedure In Microbial limit test and Biosafety

sop for Gram Staining of Bacteria in Microbiology Laboratory

sop for Monitoring of Compressed Air/gases for microbiological analysis

sop for BET (Bacterial Endotoxin) test in Microbiology

sop for receipt storage and Determining the population of Biological indicators

sop for qualification of analyst microbiologist

sop for Bioburden test of Packing materials in Microbiology Laboratory

sop for microbiological assay of erythromycin antibiotic

sop for liquid particle counter

sop for operation and calibration of digital zone reader

sop for monitoring of ultraviolet efficiency LAF and pass box

microbiological assay of cyanocobalamin or vitamin B12

gowning procedure for microbiological testing area

swab testing of various surfaces for bioburden determination

sop for endotoxin challenge test

Hold time study protocol for sterilized media

sop for personnel Qualification protocol for aseptic area

sop for sampling and testing of drain water

Sop for Operation of Airborne Particle Counter

sop for Inoculum Preparation

sop for Validation protocol of steam sterilizer autoclave

sop for pathogen detection from drain point

Sop for Analysis of Raw water Purified water water for injection and pure steam water

sop for preservatives efficacy test

sop for collection and preservation of in house isolated microorganisms

sop for Operation Calibration and Maintenance of Micropipette

Sop for UV Efficacy Test

sop for gram staining

Sop for swab testing

sop for microbiological testing of water


sop for depyrogenation of apparatus

sop for media preparation

sop for fertility test growth promotion test of media

sop for Operation and cleaning of moist heat sterilizer

sop for monitoring by active air sampler

sop for swab sampling and testing for clean rooms in production area

sop for monitoring in microbiology laboratory

sop for Fumigation of aseptic area and microbiology lab

sop for monitoring of personnel in aseptic area

sop for maintenance of cultures

sop for Operation and cleaning of laminar bench

preparation of settle plates

sop for monitoring of pure steam

sop for entry and exit procedure to m.l.t and b.e.t room

sop for storage of and use of media

sop for disposal of microbiological media and cleaning of microbiological glassware

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