sop for Receipt Storage and Usage of Culture Media

sop for Receipt Storage and Usage of Culture Media

1.1 To lay down a procedure for Receipt, Storage and Usage of Culture Media.

2.1 This procedure applicable for Receipt , Storage and Usage of Culture Media In Microbiology Laboratory

3.1 Microbiologist QC

4.1 Q. C. Manager

5.1 Receipt
5.1.1 Upon receipt of media in QC, check the media for the shipment condition.
5.1.2 Verify with the requisition, and if it tallies put a tick mark and initialize with the date of receipt.
5.1.3 Identify and circle the expiration date on the manufacturer’s label.
5.1.4 Paste the material receipt label, enter the date of receipt on the label.
5.1.5 Assign in-house expiry date (use before date) of one year from the date of opening.
5.1.6 If the manufacturer expiry date is earlier to the in-house expiry date then assign manufacturers expiry date.
5.1.7 Assign a serial number to each container.
5.1.8 Document the details of media batch no or lot no, date of receipt and the serial number allotted to each container in the inventory record.
5.1.9 If the media has to be dispensed into smaller containers, dispense the media from the bulk container to the test tube and check the pH before dispensing.

5.2 Storage
5.2.1 Store the media as directed on the manufacturer’s label. (i.e. a temperature below 25ºC in a dry place). The media for which the recommended storage temperature.
5.2.2 Protect the media from bright light.
5.3 Usage
5.3.1 Use ‘First In First Out’ system by selecting the lowest serially numbered container.
5.3.2 When a new bottle of the media is taken for use, enter the date of opening in the inventory record against the same serial number as on the container.
5.3.3 Enter the date of opening on the material receipt label.
5.3.4 Check the use before date on the container before opening the bottle.
5.3.5 Check the contents for the free flowing condition, after opening the bottle.
5.3.6 Discard the media that shows signs of hydration, cracking or discoloration.
5.3.7 Document the details in the media weighing record.
5.3.8 Calculate the required quantity of the medium on the basis of manufacturer’s instructions.
5.3.9 Weigh the calculated quantity on a calibrated balance, quickly and accurately.
5.3.10 Immediately close the bottle and tighten the lid.
5.3.11 Document the weighing in the media weighing record.
5.3.12 Add correct volume of WFI and mix thoroughly.
5.3.13 If the medium contains agar, leave the flask to stand, allow the agar particles to hydrate and then heat the media with frequent agitation, to prevent charring. Boil the media till the agar dissolves.
5.3.14 After completion of heating, add 0.5% of glycerol to Soya bean casein digest agar.
5.3.15 Dispense the media into the required containers with constant shaking.
5.3.16 Plug the container and mark every media container with media type, Date of preparation and used before Date.
5.3.17 Mark each media container for the volume level before sterilization.
5.3.18 Dispense the media into a separate screw capped tube (test tube) to check the pH.
5.3.19 Check the pH before sterilization, for liquid media at room temperature, and for agar media at 40-45 °C.
5.3.20 Adjust pH if required by using Sodium hydroxide or Hydrochloric Acid before sterilization.
5.3.21 Autoclave the media in same test tube to check the pH of the medium after sterilization and cooling.


Abbreviation used Full form of abbreviation used
SOP Standard Operating Procedure
QA Quality Assurance
QC Quality Control
SCA Soyabean Casein Digest Agar


sop for calibration and validation of micro autoclave

sop for Sterility failure investigation

cleaning and operation of discard autoclave

sop for operation of fogger machine

sop for Biological assay of lactic acid bacillus

sop for preparation of culture inoculum

STP for sterility testing of sterile gloves

sop for Operation and calibration of active air sampler

sop for transfer of material for testing and sampling in sterile area

entry & exit procedure in microbiology laboratory

Growth Promotion Test In Microbiology Laboratory

Operation of B.O.D in Microbiology Laboratory

Operation of Horizontal Laminar Air Flow in the microbiology laboratory

Operation and cleaning of Pass Box.

Operation and cleaning of air sampler

Cleaning and Sterilization of Glassware

Analysis of water for microbial load in microbiology lab

Operation and temperature monitoring of Refrigerator

Fumigation of Microbiology Laboratory.

Entry & Exit procedure In Sterility Area

SOP for Microbial analysis of Raw Material Finished Products

SOP for Operation & Calibration of pH meter in Micro Department

SOP for Operation & Calibration of pH meter in Micro Department

SOP Operation and calibration of Hot Air Oven In Microbiology

SOP for operation cleaning & calibration of Digital colony counter

SOP for Operation And Cleaning of Microscope

sop for Media Preparation and Consumption

sop for Receipt Storage and Usage of Culture Media

sop for Cleaning Sanitization And Disinfection In Microbiology

sop for Environmental monitoring of all the Classified area

sop for Handling and Sub culturing of Microbial cultures

sop for Media Growth Promotion Test and various Microbiological test

sop for BOD incubator operation and cleaning

sop sampling of water for microbiological analysis

sop for Disinfectant Efficacy Test

sop for for cleaning and operation of vortex mixture

sop for Temperature & Relative Humidity Monitoring

sop for Operation and Calibration of Heating Block

sop for Sterility Testing of Microbiology

sop for Disposal of Culture Media

sop for Drain point of Microbiology

sop for entry & exit procedure In Microbial limit test and Biosafety

sop for Gram Staining of Bacteria in Microbiology Laboratory

sop for Monitoring of Compressed Air/gases for microbiological analysis

sop for BET (Bacterial Endotoxin) test in Microbiology

sop for receipt storage and Determining the population of Biological indicators

sop for qualification of analyst microbiologist

sop for Bioburden test of Packing materials in Microbiology Laboratory

sop for microbiological assay of erythromycin antibiotic

sop for liquid particle counter

sop for operation and calibration of digital zone reader

sop for monitoring of ultraviolet efficiency LAF and pass box

microbiological assay of cyanocobalamin or vitamin B12

gowning procedure for microbiological testing area

swab testing of various surfaces for bioburden determination

sop for endotoxin challenge test

Hold time study protocol for sterilized media

sop for personnel Qualification protocol for aseptic area

sop for sampling and testing of drain water

Sop for Operation of Airborne Particle Counter

sop for Inoculum Preparation

sop for Validation protocol of steam sterilizer autoclave

sop for pathogen detection from drain point

Sop for Analysis of Raw water Purified water water for injection and pure steam water

sop for preservatives efficacy test

sop for collection and preservation of in house isolated microorganisms

sop for Operation Calibration and Maintenance of Micropipette

Sop for UV Efficacy Test

sop for gram staining

Sop for swab testing

sop for microbiological testing of water


sop for depyrogenation of apparatus

sop for media preparation

sop for fertility test growth promotion test of media

sop for Operation and cleaning of moist heat sterilizer

sop for monitoring by active air sampler

sop for swab sampling and testing for clean rooms in production area

sop for monitoring in microbiology laboratory

sop for Fumigation of aseptic area and microbiology lab

sop for monitoring of personnel in aseptic area

sop for maintenance of cultures

sop for Operation and cleaning of laminar bench

preparation of settle plates

sop for monitoring of pure steam

sop for entry and exit procedure to m.l.t and b.e.t room

sop for storage of and use of media

sop for disposal of microbiological media and cleaning of microbiological glassware

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