sop for Operation and Calibration of Heating Block

sop for Operation and Calibration of Heating Block



1.1 To lay down the procedure for Operation and Calibration of Heating Block.


2.1 This procedure applicable for Operation and Calibration of Heating Block for Microbiology Laboratory


3.1 Microbiologist QC


4.1 QC Manager


5.1 Requirement
5.1.1 Heating block
5.2 Procedure
5.2.1 Connect the power cord of the heating block to the electrical main switch.
5.2.2 Switch ON the mains and switch ON the “Power” switch present on front panel of the Heating block.
5.2.3 To change the set temperature press red coloured “Press to read set temperature” push button & simultaneously

rotate the “Coarse” and “Fine” knob in clockwise or anticlockwise direction to increase or decrease the set temperature, respectively.
5.2.4 Set the “Heater Capacity” Knob at 37°C (±1°C).
5.2.5 Wait for the temperature to reach the set temperature and stabilize.
5.2.6 Place the assay tubes into the wells for incubation for the specified time.
5.2.7 After the incubation, remove the test tubes and switch OFF the instrument by pressing the “Power” switch.
5.2.8 Record the operation of heating block log book – Annexure-1.
5.3.1 Clean the external surface of the heating block with lint free wet cloth followed by the 70% IPA mop.
5.3.2 Frequency: As and when required.

5.4.1 Place the Heating block on a vibration free surface.
5.4.2 When the incubation is in process do not disturb the heating block or the table on which it is placed.
5.5.1 In case of any discrepancy affix “Out Of Order” tag with sign & date and inform Engineering department for rectification.
5.5.2 If temperature is found out of range then check, set temperature and if required.
INTERNAL: Calibration shall be done quarterly.
EXTERNAL: Shall be done once in a year.

Abbreviation used Extended Form
SOP Standard Operating Procedure
AHU Air Handling Unit
IPA Iso-Propyl Alcohol

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sop for for cleaning and operation of vortex mixture

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sop for Operation and Calibration of Heating Block

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swab testing of various surfaces for bioburden determination

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Hold time study protocol for sterilized media

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sop for Validation protocol of steam sterilizer autoclave

sop for pathogen detection from drain point

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sop for storage of and use of media

sop for disposal of microbiological media and cleaning of microbiological glassware

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