cleaning and operation of discard autoclave



cleaning and operation of discard autoclave


1.1 To describe a procedure for the operation and cleaning of Discard autoclave in microbiology laboratory.
2.1 This procedure is applicable for the operation of discard autoclave which is used for discarding used media from various testing in Microbiology Laboratory
3.1 Officer Microbiologist
3.2 Executive Microbiologist

4.1 Head – QC

5.1 Material Required
5.1.1 Used Culture Media, Autoclavable polybags, Sterilization indicator strips, sterile gloves, face mask.
5.2 Equipments Required
5.2.1 Discard Autoclave

5.3 Operation:

5.3.1 Ensure the level of water in autoclave is sufficient.
5.3.2 Put the Media bin into autoclave and cover the lid of autoclave along with sterilization indicator strip.
5.3.3 Adjust all the six clamps on autoclave lid and then close the clamps by tightening their knobs properly.
5.3.4 Ensure that the steam release valve is closed.
5.3.5 Ensure that the pressure gauge is showing zero pressure.
5.3.6 Turn “ON” the power switch.

5.3.7 Note down the start time of autoclave in record.

5.3.8 Slowly release the air from autoclave when pressure raises up to 5 psi.

5.3.9 When the pressure reaches to 15 psi note down the time and let the process run for 30 minutes from this time.
5.3.10 After 30 minutes turn of the power switch of autoclave and slowly release the steam of autoclave by opening the steam release valve.
5.3.11 After “END OF PROCESS” when pressure reaches to the ZERO, Wear protective latex gloves and face mask and open the lid of autoclave. Remove the sterilized items and keep at proper place.

5.3.12 Take out the sterilization indicator from autoclave and check the status of sterilization with the help of color indication on indicator and attach the indicator strip in record.
5.4 Precaution

5.4.1 Don’t touch the external surface of autoclave during its sterilization cycle.
5.4.2 Wear latex gloves and face mask while unloading the material.

5.4.3 Remove the air from autoclave by opening the steam release valve when the pressure rises to 5 psi.
5.4.4 After closing the autoclave lid, check that the lid is locked properly so that it does not get opened by the pressure.
5.4.5 If the safety valve does not release steam, and if pressure rises above 20 psi, turn the autoclave off immediately.

5.4.6 Before loading ensure that autoclave chamber is clean and water level is sufficient.
5.4.7 Always remove air from chamber after starting the autoclave by opening the steam release valve when pressure rises up to 5psi.
5.5 Cleaning
5.5.1 Ensure the power supply is off before starting cleaning operation.

5.5.2 Deduct the autoclave from outside.
5.5.3 Ensure the chamber surfaces are at a temperature below 40 0C.

5.5.4 Open the autoclave and remove the trays from the autoclave chamber.

5.5.5 Before starting the operations, clean the chamber, door and inner surfaces with the scrubber and wash thoroughly with purified water.

5.5.6 Allow the water to drain.

5.5.7 Wipe the cleaned surfaces with dry cloth.
5.5.8 Remove the drain strainer, clean if any residual fibers, lint or sedimentation is formed in the pores.

Frequency for Cleaning – weekly


6.1 Nil
7.1 In House
Discard Autoclave Sterilization Record
9.1 QC : Quality Control
9.2 QCM. : Quality Control Microbiology

9.3 SOP : Standard Operating Procedure
9.4 QA : Quality Assurance
9.5 PSI : Per square inch
9.6 IPA : Iso Propyl Alcohol


discard autoclave sterilization record




Lot No.

Sterilization                               temperature




Start Time


End Time

Sterilization Hold

Done By




sop for calibration and validation of micro autoclave

sop for Sterility failure investigation

cleaning and operation of discard autoclave

sop for operation of fogger machine

sop for Biological assay of lactic acid bacillus

sop for preparation of culture inoculum

STP for sterility testing of sterile gloves

sop for Operation and calibration of active air sampler

sop for transfer of material for testing and sampling in sterile area

entry & exit procedure in microbiology laboratory

Growth Promotion Test In Microbiology Laboratory

Operation of B.O.D in Microbiology Laboratory

Operation of Horizontal Laminar Air Flow in the microbiology laboratory

Operation and cleaning of Pass Box.

Operation and cleaning of air sampler

Cleaning and Sterilization of Glassware

Analysis of water for microbial load in microbiology lab

Operation and temperature monitoring of Refrigerator

Fumigation of Microbiology Laboratory.

Entry & Exit procedure In Sterility Area

SOP for Microbial analysis of Raw Material Finished Products

SOP for Operation & Calibration of pH meter in Micro Department

SOP for Operation & Calibration of pH meter in Micro Department

SOP Operation and calibration of Hot Air Oven In Microbiology

SOP for operation cleaning & calibration of Digital colony counter

SOP for Operation And Cleaning of Microscope

sop for Media Preparation and Consumption

sop for Receipt Storage and Usage of Culture Media

sop for Cleaning Sanitization And Disinfection In Microbiology

sop for Environmental monitoring of all the Classified area

sop for Handling and Sub culturing of Microbial cultures

sop for Media Growth Promotion Test and various Microbiological test

sop for BOD incubator operation and cleaning

sop sampling of water for microbiological analysis

sop for Disinfectant Efficacy Test

sop for for cleaning and operation of vortex mixture

sop for Temperature & Relative Humidity Monitoring

sop for Operation and Calibration of Heating Block

sop for Sterility Testing of Microbiology

sop for Disposal of Culture Media

sop for Drain point of Microbiology

sop for entry & exit procedure In Microbial limit test and Biosafety

sop for Gram Staining of Bacteria in Microbiology Laboratory

sop for Monitoring of Compressed Air/gases for microbiological analysis

sop for BET (Bacterial Endotoxin) test in Microbiology

sop for receipt storage and Determining the population of Biological indicators

sop for qualification of analyst microbiologist

sop for Bioburden test of Packing materials in Microbiology Laboratory

sop for microbiological assay of erythromycin antibiotic

sop for liquid particle counter

sop for operation and calibration of digital zone reader

sop for monitoring of ultraviolet efficiency LAF and pass box

microbiological assay of cyanocobalamin or vitamin B12

gowning procedure for microbiological testing area

swab testing of various surfaces for bioburden determination

sop for endotoxin challenge test

Hold time study protocol for sterilized media

sop for personnel Qualification protocol for aseptic area

sop for sampling and testing of drain water

Sop for Operation of Airborne Particle Counter

sop for Inoculum Preparation

sop for Validation protocol of steam sterilizer autoclave

sop for pathogen detection from drain point

Sop for Analysis of Raw water Purified water water for injection and pure steam water

sop for preservatives efficacy test

sop for collection and preservation of in house isolated microorganisms

sop for Operation Calibration and Maintenance of Micropipette

Sop for UV Efficacy Test

sop for gram staining

Sop for swab testing

sop for microbiological testing of water


sop for depyrogenation of apparatus

sop for media preparation

sop for fertility test growth promotion test of media

sop for Operation and cleaning of moist heat sterilizer

sop for monitoring by active air sampler

sop for swab sampling and testing for clean rooms in production area

sop for monitoring in microbiology laboratory

sop for Fumigation of aseptic area and microbiology lab

sop for monitoring of personnel in aseptic area

sop for maintenance of cultures

sop for Operation and cleaning of laminar bench

preparation of settle plates

sop for monitoring of pure steam

sop for entry and exit procedure to m.l.t and b.e.t room

sop for storage of and use of media

sop for disposal of microbiological media and cleaning of microbiological glassware




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