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sop for microbiological testing of water

Sop for microbiological testing of water

1 OBJECTIVE
To describe the procedure for analysis of water sample for estimation of the number of viable aerobic micro-organisms present & for the detection of Pathogenic microbial species.

2 SCOPE
This procedure is applicable to the analysis of water for microbial load in microbiology lab

3 RESPONSIBILITY
Microbiologist.

4 ACCOUNTABILITY
Head – Q. C

5 ABBREVIATIONS
SOP : Standard Operating Procedure
Q .A. : Quality Assurance
Q. C. : Quality Control
NO. : Number
MLT : Microbial limit test
SCDM : Soyabean Casein Digest Medium.
HLAF : Horizontal laminar air flow
DM : De-mineralized.
WFI : Water for Injection.



6 PRECAUTIONS
6.1 Water sampling should be done in sterile screw cap bottles following the SOP No.
6.2 Sample the water aseptically in sampling bottle.
6.3 Media should be fresh for use in test.

7 MATERIALS REQUIRED
Soyabean Casein Digest Medium, R-2A media, Peptone water, Sterile Petri plates, test tubes, Micropipette (1ml), Micro tips (1ml), Filtration assemblies, Sterile Gridded nitrocellulose Filter paper (0.45 μ),
Sterile forceps, Sterile scissor, Filtered 70%IPA, Selective media as per requirement.

8 EQUIPMENTS REQUIRED
HLAF, Autoclave, Incubators, Burner, Colony counter, Inoculating loop.

9 PROCEDURE
9.1 Total Aerobic Microbial Count
9.1.1. Plate count method: (Potable Water)
9.1.1.1 Take 1.0 ml of the sample in sterilized Petri plate.
9.1.1.2 Pour 15.0 to 20.0 ml of autoclaved and cooled to 45 to 48°C of R2A agar medium.
9.1.1.3 Mix the contents of the Petri plate by rotating it gently and allow the media to solidify.
9.1.1.4 Invert the Petri plates and incubate in BOD incubator at 30˚C to 35˚C for 5 days for bacterial
and fungal count.
9.1.1.5 After incubation examine the Petri plates for growth and count the number of colonies by using
Colony counter.
9.1.1.6 01ml Sample in 09ml Soyabean casein Digest Medium mix well and keep at 20 to 25ºC for about 2 to5
hours to resuscitate the organism.
9.1.1.7 01ml inoculated prepared sample inoculate in 09ml Enterobacteria Enrichment Broth-Mossel and
incubate at 30 to 35ºC for 24 to 48 hours and subculture Violet Red Bile Glucose Agar incubate at 30
to 35ºC for 18 to 24 hours. Sample is passed if there is no growth on media plates.
9.1.2. Membrane filtration method     (WFI and Purified Water)
9.1.2.1 Filtered 200ml of WFI & 1ml Purified water sample inoculate to100ml sterile water/peptone, from
water sampling bottle.
9.1.2.2 Remove butter paper from filtration assembly and silicon tubing under HLAF.
9.1.2.3 Connect filtration assembly to the filtration flask and vacuum pump with the help of silicon tubing.
9.1.2.4 Take out the sterile 0.45μ membrane filter from the individual pack and place the filter paper
sandwiched between filtration cup & filtration receptacle with the help of sterile forcep.
9.1.2.5 Open the lid of filtration cup filter the approx 15-20ml sterile peptone water.
9.1.2.6 Take water sample collected in sampling bottle, open the lid carefully without touching.
9.1.2. 7 Switch “ON” the vacuum pump. Open the lid of filtration cup & pour the content of sampling bottle
into the filtration cup.
9.1.2.8 Switch off the vacuum pump and remove the SS receptacle from the holder base aseptically.
9.1.2.9 By means of sterile forcep transfer the filter paper on the R2A agar plate aseptically.
9.1.2.10 Incubate the plate at 30˚C to 35˚C for 5 days.
9.1.2.11 Complete incubation period, observe the colonies and count by using colony Counter.
9.2. Pathogens testing
9.2.1 Prepare 100ml SCDM tube and all selective media as per SOP for media preparation
(EH/MIC/SOP/018)



9.2.2. Test for E.coli
9.2.2.1 Enrichment:
Filter the 100ml of water sample and transfer the filter paper very carefully in 100ml Soybean Casein
Digest Medium, shake gently and incubate at 30°C -35°C for 18 to 24 hrs. After incubation examine the tube. If growth is present mix by gentle shaking. Further proceed for the specified test.

9.2.2.2 Primary Test:
Shake the tube, transfer 1.0 ml of soyabean casein digest broth into bottle containing 100ml of
MacConkey broth and incubate at 42 C to 44˚C for 24 to 48 hrs.
9.2.2.3 Secondary Test:
Subculture on the surface of MacConkey agar plate and incubate at 30°C to 35°C for 18 to 72 hrs. If
Appearance of pinkish red color colonies on Mac Conkey agar indicates the presence of E.coli.
9.2.2.4 Confirmatory Test: Indole Test:
Inoculate the suspected colonies to test tube containing 5.0 ml peptone water. Incubate the tube at
30°C -35°C for 24 hrs, for indole production. Add 0.5 ml Kovac’s reagent shake well and allow to stand
for one minute if red colour ring is observed in the reagent layer indole is present.
MORPHOLOGICAL CHARACTERITICS OF E.coli SPECIES ON
SELECTIVE CULTURE MEDIA TABLE- I

MEDIUM DESCRIPTION OFCOLONY
Mac Conkey agar

Mac Conkey broth

Kovac’s reagent

Pinkish red colour colonies may have surrounding zone of precipitated bile

Growth present

Red ring formation

9.2.3 Test for Salmonella
9.2.3.1 Enrichment
Filter the 100ml of water sample and transfer the filter paper very carefully in 100ml Soybean Casein
Digest Medium, shake gently and incubate at 30°C to 35°C 18 to 24 hrs. After incubation examine
the tube. If growth is present mix by gentle shaking. Further proceed for the specified test.
9.2.3.2 Primary Test:
Add 0.1 ml of enrichment culture transfer test tube containing 10 ml Rappaport Vassiliadis
Salmonella Enrichment Broth and incubate at 30˚C to 35˚C for 24 to 48 hrs. After incubation
the tube subculture on the Xylose Lysine Deoxycholate Agar and incubate at 30˚C to 35˚C for 24 to
48 hrs. If red colonies with or without black centers possibly of salmonella.
9.2.3.3 Secondary Test:
Subculture those colonies which show the characteristics as given in table II. Subculture the triple
sugar iron agar slants by stabbing the wire well beneath the surface. Incubate the stabbed slants at
30°C to 35°C for 24 hrs. The formation of acid and gas in the stab culture (with or without Concomit –
-ant blackening) confirms the presence of Salmonella while its absence confirms the specimen or
sample to be free from Salmonella.
MORPHOLOGICAL CHARACTERITICS OF SALMONELLA SPECIES ON
SELECTIVE CULTURE MEDIA TABLE-II

MEDIUM DESCRIPTION OF COLONY
Xylose Lysine Deoxycholate Agar Red with or without black centers

9.2.4 Test for Pseudomonas aeruginosa
9.2.4.1 Enrichment: Filter the 100ml of water sample and transfer the filter paper very carefully in 100ml.Soybean Casein Digest Medium, shake gently and incubate at 30°C to 35°C 18 to 24 hrs. After incubation examine the tube. If growth is present mix by gentle shaking. Further proceed for the specified test.
9.2.4.2 Primary Test: Streak one loop full of the enrichment culture on the surface of Cetrimide agar plate, and incubate the plates in inverted position at 30 to 35 ˚C for 18 to 72 hrs. Greenish color colonies indicate the possibility of Pseudomonas aeruginosa
9.2.4.3 Oxidase test: Place 2 or 3 drops of Oxidase reagent (Tetra methyl para phenylene diammonium dihydro-Chloride) on a piece of filter paper (WhatmanNo.1) and transfer the suspected colony making smear on the paper if a pink colour is produced changing to purple within 5 –10 seconds, the test confirms the presence of P. aeruginosa.

MORPHOLOGICAL CHARACTERITICS OF SALMONELLA SPECIES ON
SELECTIVE CULTURE MEDIA Table – III

Medium Characteristics colonial morphology Fluorescence in UV light
Cetrimide agar Generally greenish Greenish

9.2.5 Test for Staphylococcus aureus 9.2.5.1Enrichment: Filter the 100ml of water sample and transfer the filter paper very carefully in 100ml
Soybean Casei Digest Medium, shake gently and incubate at 30°C to 35°C for 18 to 24 hrs. After
incubation examine the tube. If growth is present mix by gentle shaking. Further proceed for the
specified test.
9.2.5.2 Primary Test:Streak one loop full of the enrichment culture on the surface of Mannitol Salt agar incubate the plates inverted position at 30°C to 35°C for 18 to 72 hrs. Yellow or white colonies with yellow zones indicate the possibility of presence of Staphylococcus aureus. Proceed further for confirmatory test.
9.2.5.3 Confirmatory Test Coagulase Test:
With the help of inoculating loop transfer representative suspected colonies from the agar surface of
the Vogel Johnson agar or Mannitol salt agar to individual tube containing 0.5 ml mammalian plasma
preferably rabbit or horse plasma with or without suitable additive. Incubate in a water bath at 30°C to
35°C and three hours and subsequently at suitable intervals up to 24hrs. along with test positive and
negative control simultaneously. If no coagulation in any degree is observed, the specimen meets the
requirements of the test of absence of Staphylococcus aureus

 

MORPHOLOGICAL CHARACTERITICS OF SALMONELLA SPECIES ON
SELECTIVE CULTURE MEDIA TABLE- IV

    MEDIUM DESCRIPTION OF COLONY
Mannitol Salt agar Yellow colonies with yellow zones

9.2.6 Test for bile tolerant gram negative bacteria test:
9.2.6.1 Enrichment: 01 ml sample in 09 ml Soyabean casein digest Medium mix well and keep at 20 to 25ºc for about 2 to 5 hours.
9.2.6.2 Primary test: 01 ml inoculated prepared sample inoculate in 09 ml Enterobacteria Broth Mossel incubate at 30 to 35ºC for 24 to 48 hours. Sub culture on violet Red Bile Glucose agar incubate 30 to 35ºC for 18 to 24 hours. Sample is passed if there is no growth on media pltes.
9.2.6.3 Secondary Test:(if growth observed on media plates then carry out the quantitative test)
01 ml sample in 09 ml soyabean casein digest medium mix well and keep at 20 to 25ºC for about 2 to 5 hours. Take volume corresponding to 0.1 ml,0.01 ml,0.001ml of the sample suitable quantity in Enterobacteria Broth Mossel incubate at 30 to 35ºC for 24 to 48 hours. Sub culture on violet Red Bile Glucose agar incubate 30 to 35ºC for 18 to 24 hours. Sample quantity determined as per the table.

Result for each quantity of sample Number of Bacteria per ml of Sample
0.1ml 0.01ml 0.001ml  

More than 10³

Less than 10³ and more than 10²

Less than 10² and more than 10

Less than 10

  +

+

+

    +

+

       +

WATER LIMIT:

Alert Limit Action Limit Specification Limit
RAW WATER 300cfu/ml 400 cfu/ml 500 cfu/ml
PURIFIED WATER 50 cfu/ml 80 cfu/ml 100 cfu/ml
WATER FOR INJECTION 05 cfu/100ml 08 cfu/100ml 10 cfu/100ml
PATHOGENS Should be absent/100 ml
Bile Tolerant Gram negative Bacteria Should be absent/ ml

Remark: Alert Limit:- If the alert limit is crossed then immediately inform to QA department and water generation department. Increase the sampling frequency, monitor the trend until proper preventive action is taken.
Action Limit :- If action limit is crossed then immediately inform to QA department and water generation department. Immediately stop the production and drain water from water storage tank. Sanitize the water for injection storage tank, investigate the cause, after sanitization, and collect the sample for testing, until satisfactory result are obtained (sanitize the complete water system if required) After that, intimate to the water generation department to produce the water for regular production.

10. FREQUENCY
Daily

11. ENCLOSURES
ANNEXURE -1 Raw /Treated /Purified Water Analysis Report
ANNEXURE -2 Water For Injection Analysis Report
ANNEXURE -3 Change History Log Format

12. REFERENCE DOCUMENTS
Pharmacopoeias (IP/BP/USP)

 

 

 

ANNEXURE -1

QUALITY CONTROL DEPARTMENT (MICROBIOLOGY)



RAW/PURIFIED WATER ANALYSIS REPORT

1- TOTAL VIABLE AEROBIC COUNT:
Purified Water: 1.0 ml of water sample inoculate to100 ml sterile water/ Peptone water shake well and filter
Through 0.45μm filter paper and put on the R2A Agar plates and incubate at 30°C to 35°C for 5 Days.

Raw Water: Take1.0ml of water sample and pour in to sterile Petri plate. Pour 15-20ml sterilized R2A (temp.
Should not more than 50oC), mix by gentle rotating and incubate after media get solidified.
Medium : R2A Agar Autoclave Lot. No.:
Batch No. : Peptone Autoclave lot No.:
Incubation : 30°C to 35°C for 5 Days
Observation: Sampling point No. _________cfu/ml
Sampling point No. _________cfu/ml
Sampling point No. _________cfu/ml Sign:
Negative Control: Growth observed/not observed. Date:
2- PATHOGEN TESTING:
Filter_____ml water sample and transfer filter paper carefully in ______ml Soyabean Casein Digest Medium (Soln A) Incubate at 30°C to 35°C for 24 hrs.
Test for Escherichia coli:-
a) Primary Test:1ml of soln A transfer in 100 ml MacConkey Broth Incubate at 42°C to 44°C for 24 to 48 hrs.
i) MacConkey Broth: Autoclave Lot. No.
Observation:
Positive Control: Growth observed Observed/not observed
Negative Control: Growth observed Observed/not observed
Sampling Point No.: Growth observed Observed/not observed
Sampling Point No.: Growth observed Observed/not observed
Sampling Point No.: Growth observed Observed/not observed
b)Secondary Test : Sign: Date:
Subculture on the surface of MacConkey agar plate and incubate at 30°C to 35°C for 18 to 72 hrs.
MacConkey agar: Autoclave Lot. No.
Observation:
Positive Control: Pinkish red colour colonies with zone of precipitated bile Observed/not observed
Negative Control Pinkish red colour colonies with zone of precipitated bile Observed/not observed
Sampling Point No Pinkish red colour colonies with zone of precipitated bile Observed/not observed
Sampling Point No Pinkish red colour colonies with zone of precipitated bile Observed/not observed
Sampling Point No Pinkish red colour colonies with zone of precipitated bile Observed/not observed
Sign: Date:
c) Confirmatory Test    If positive characters observed then carry out the confirmatory test)
Inoculate the suspected colonies to 5 ml Peptone Water. Incubate at 40-45°C for 24 hrs. After Incubation
add 3-5 drops of Kovac’s, reagent. Carry the positive control test using E.coli culture to both tubes.
Observation Positive Control: Red color ring Observed/ not observed
Negative Control: Red color ring Observed/ not observed
Sampling Point No.: ______ Red color ring Observed/ not observed
Sampling Point No.: ______ Red color ring Observed/ not observed
Sampling Point No.: ______ Red color ring Observed/ not observed
Test for Salmonella Species Sign: Date:
a) Primary test: 0.1 ml of Soln A to 10 ml Rappaport Vassiliadis Salmonella Enrichment Broth and incubate
at 30°C to 35°C for 24 to 48 hrs. After incubation subculture on the surface of Xylose lysine deoxycholate agar and incubate the Petri plates at 30°C to 35°C for 24 to 48hrs.
i) Rappaport Vassiliadis Salmonella Enrichment Broth: Autoclave Lot. No.
Observation: Positive Control: Growth Observed/ not observed
Negative Control: Growth Observed/ not observed
Sampling Point No.: ______ Growth Observed/ not observed
Sampling Point No.: ______ Growth Observed/ not observed
Sampling Point No.: ______ Growth Observed/ not observed Sign: Date:
ii) Xylose Lysine Deoxycholate Agar: Autoclave Lot. No.
Observation: Positive Control:Red with or without black centered colonies Observed/not observed
Negative Control: Red with or without black centered colonies Observed/not observed
Sampling Point No.: ______ Red with or without black centered colonies Observed/not observed
Sampling Point No.: ______ Red with or without black centered colonies Observed/not observed
Sampling Point No.: ______ Red with or without black centered colonies Observed/not observed
Sign: Date:
b) Secondary Test: (If positive characters observed then carry out the secondary test)
If typical growth observed then subculture on TSI slant and incubate at 30-35°C for 24 hrs.
Triple Sugar Iron (TSI) Agar Slant: Autoclave Lot. No.
Observation:
Positive Control: Pink slants with yellow butt and gas Observed/not observed
Negative Control: Pink slants with yellow butt and gas Observed/not observed
Sampling Point No.:______ Pink slants with yellow butt and gas Observed/not observed
Sampling Point No.:______ Pink slants with yellow butt and gas Observed/not observed Sign:
Sampling Point No.:______ Pink slants with yellow butt and gas Observed/not observed Date:

Test for Pseudomonas aeruginosa:
a) Primary Test:
If turbidity is observed in the (Sol. A), streak a loopfull from soln. A on cetrimide agar & Pseudomonas
Agar plate & incubate at 30°C to 35°C for 18 to72 hrs.
i) Cetrimide Agar Plate: Autoclave Lot. No.:
Observation
Positive Control: greenish colonies/ Greenish under UV light Observed/not observed
Negative Control: greenish colonies/ Greenish under UV light Observed/not observed
Sampling Point No.:______ greenish colonies/ Greenish under UV light Observed/not observed
Sampling Point No.:______ greenish colonies/ Greenish under UV light Observed/not observed
Sampling Point No.: ______ greenish colonies/ Greenish under UV light Observed/not observed
Sign: Date: b) Secondary Test:( If positive characters observed then carry out the secondary test)
Oxidase Test :
Positive Control: Purple colour Observed/ not observed
Sampling Point No.:______ Purple colour Observed/ not observed
Sampling Point No.: ______ Purple colour Observed/ not observed
Sampling Point No.: ______ Purple colour Observed/ not observed

Test for Staphylococcus aureus:
a) Primary Test:
If turbidity observed in the (Soln. A), streak a loop full from soln. A on Mannitol Salt Agar Incubate at 30°C to 35°C for 18 – 72 hrs.
i) Mannitol Salt Agar: Autoclave Lot. No.:
Observation:
Positive Control: Yellow colonies with yellow zones Observed/not observed
Negative Control: Yellow colonies with yellow zones Observed/not observed
Sampling Point No.:______ Yellow colonies with yellow zones Observed/not observed
Sampling Point No.:______ Yellow colonies with yellow zones Observed/not observed
Sampling Point No.:______ Yellow colonies with yellow zones Observed/not observed
Sign: Date:
b) Secondary Test: Coagulase Test (If positive characters observed then carry out the secondary test)
Transfer representative suspected colonies from the agar surface of above Medium to the tubes,
each containing 0.5 ml of mammalian plasma (preferably rabbit or horse). Incubate in water bath at
37°C; examine the tubes at 3 hrs and subsequently at suitable interval up to 24 hrs.
Observation:
Positive control: Coagulation observed/not observed
Negative control: Coagulation observed/not observed
Sampling Point No.:______ Coagulation observed/not observed
Sampling Point No.:______ Coagulation observed/not observed
Sampling Point No.:______ Coagulation observed/not observed
Sign: Date:

Test for Bile Tolerant Gram Negative Bacteria test:
a) Primary Test —–ml sample in —–ml Soyabean Casein digest Medium mix and keep at 20 to 25ºC for about 20to 5 hours.
—-ml inoculated prepared sample inoculate in —-ml Enterobacteria Enrichment Broth-Mossel incubate at 30 to 35ºC for 24 to 48 hours.
Sub culture on Violet Red Bile Glucose Agar incubate at 30 to 35ºC for 18 to 24 hours. Sample is passed if there is no growth on media plates.
Observation:
Positive Control: reddish colonies Observed/Not observed
Negative Control: reddish colonies Observed/Not observed
Sampling Point No: reddish colonies Observed/Not observed
Sampling Point No: reddish colonies Observed/Not observed
b) Secondary Test: (If growth observed on media plates then carry out the Quantitative test)
—-ml sample in —ml Soyabean casein digest Medium mix well and keep at 20 to 25ºC for about 2 to 5 hours. Take volume corresponding to 0.1ml, 0.01ml, 0.001ml of the sample suitable quantity Enterobacteria Enrichment Broth-Mossel incubates at 30 to 35ºC for 18 to 24 hours. Sample quantity determine as per the table.

 

 

Result for each quantity of sample Number of Bacteria per ml of Sample
0.1ml 0.01ml 0.001ml  

More than 10³

Less than 10³ and more than 10²

Less than 10² and more than 10

Less than 10

  +

+

+

    +

+

       +

Observation :
Sampling Point No:————- cfu/ml Observed. Sing:
Sampling Point no:————- cfu/ml Observed Date:

 

RESULT:

TOTAL VIABLE COUNT RESULT REMARKS SIGN./DATE
 

Sampling Point………

 

Sampling Point………

 

Sampling Point………

Bacterial                    Cfu/ml

Fungal                       Cfu/ml

 

Bacterial                  Cfu/ml

Fungal                     Cfu/ml

 

Bacterial                   Cfu/ml

Fungal                     Cfu/ml

 

Complies/does not comply

 

 

Complies/does not comply

 

 

Complies/does not comply

Sr. No. PATHOGEN SampleSpot NO. Observation REMARK SIGN./DATE
 

1.

 

E.coli

 

 

 

Detected/ Not detected

Detected/ Not detected

Detected/ Not detected

Complies/ not comply

Complies/ not comply

Complies/ not comply

 

2.

 

Salmonella

 

 

 

Detected/ Not detected

Detected/ Not detected

Detected/ Not detected

Complies/ not comply

Complies/ not comply

Complies/ not comply

 

3.

 

Pseudomonas aeruginosa

 

 

 

Detected/ Not detected

Detected/ Not detected

Detected/ Not detected

Complies/ not comply

Complies/ not comply

Complies/ not comply

 

4.

 

Staphylococcus aureus

 

 

 

Detected/ Not detected

Detected/ Not detected

Detected/ Not detected

Complies/ not comply

Complies/ not comply

Complies/ not comply

5. Bile- Tolerant Gram Negative Bacteria Detected/ Not detected

Detected/ Not detected

Detected/ Not detected

Complies/ not comply

Complies/ not comply

Complies/ not comply

Alert Limit Action Limit Specification Limit
RAW WATER 300cfu/ml 400 cfu/ml 500 cfu/ml
PURIFIED ( DM ) WATER 50 cfu/ml 80 cfu/ml 100 cfu/ml
PATHOGENS Should be absent/100 ml
Bile Tolerant Gram negative Bacteria Should be absent/ ml

CONCLUSION: The above water sample complies/does not complies to the specifications.

Observed By Reviewed By Approved By

ANNEXURE -2

QUALITY CONTROL DEPARTMENT (MICROBIOLOGY)

WATER FOR INJECTION ANALYSIS REPORT

Report No. :

SAMPLING DETAILS                                                              INCUBATION DETAILS

Date of  Sampling      :                                                      Incubation Temperature &     :   30oC – 35oC for 5 days

Quantity  Sampled     :                                                      Incubation  Period

Sampled  By              :                                                     EQUIPMENT DETAILS

Date of  Analysis       :                                                       BOD Incubator ID No.   :  EH/EQ/MIC/BOD-03

Analyzed  By             :

 

Media used  :R2A aga                                                                                             Method  Details

Batch No.     :                                                                   Method Used                 : Membrane Filtration Method

Autoclave lot no:                                                              SOP No.                         :

 

OBSERVATIONS

Sampling Point Location Sampling

Point  No

Microbial counts per 100 ml REMARK
  BACTERIAL COUNT

+Ve Control = CFU Present / Absent -Ve Control = CFU Present / Absent

Note :- A = Absent, P = Present

LIMITS –

Alert Limit Action Limit Specification Limit
WATER FOR INJECTION 05 cfu/100ml 08 cfu/100ml 10 cfu/100ml
PATHOGENS Should be absent/100 ml

Remarks: The sample under analysis Complies / Does not Comply as per the Standard Limits.

 

Observed By                                                                                      Reviewed By                                                                                Approved By

 

ANNEXURE-3

CHANGE HISTORY LOG FORMAT

Rev. No. Details of changes Reason for change Effective Date Updated By
 

 

 

 

 

sop for calibration and validation of micro autoclave

sop for Sterility failure investigation

cleaning and operation of discard autoclave

sop for operation of fogger machine

sop for Biological assay of lactic acid bacillus

sop for preparation of culture inoculum

STP for sterility testing of sterile gloves

sop for Operation and calibration of active air sampler

sop for transfer of material for testing and sampling in sterile area

entry & exit procedure in microbiology laboratory

Growth Promotion Test In Microbiology Laboratory

Operation of B.O.D in Microbiology Laboratory

Operation of Horizontal Laminar Air Flow in the microbiology laboratory

Operation and cleaning of Pass Box.

Operation and cleaning of air sampler

Cleaning and Sterilization of Glassware

Analysis of water for microbial load in microbiology lab

Operation and temperature monitoring of Refrigerator

Fumigation of Microbiology Laboratory.

Entry & Exit procedure In Sterility Area

SOP for Microbial analysis of Raw Material Finished Products

SOP for Operation & Calibration of pH meter in Micro Department

SOP for Operation & Calibration of pH meter in Micro Department

SOP Operation and calibration of Hot Air Oven In Microbiology

SOP for operation cleaning & calibration of Digital colony counter

SOP for Operation And Cleaning of Microscope

sop for Media Preparation and Consumption

sop for Receipt Storage and Usage of Culture Media

sop for Cleaning Sanitization And Disinfection In Microbiology

sop for Environmental monitoring of all the Classified area

sop for Handling and Sub culturing of Microbial cultures

sop for Media Growth Promotion Test and various Microbiological test

sop for BOD incubator operation and cleaning

sop sampling of water for microbiological analysis

sop for Disinfectant Efficacy Test

sop for for cleaning and operation of vortex mixture

sop for Temperature & Relative Humidity Monitoring

sop for Operation and Calibration of Heating Block

sop for Sterility Testing of Microbiology

sop for Disposal of Culture Media

sop for Drain point of Microbiology

sop for entry & exit procedure In Microbial limit test and Biosafety

sop for Gram Staining of Bacteria in Microbiology Laboratory

sop for Monitoring of Compressed Air/gases for microbiological analysis

sop for BET (Bacterial Endotoxin) test in Microbiology

sop for receipt storage and Determining the population of Biological indicators

sop for qualification of analyst microbiologist

sop for Bioburden test of Packing materials in Microbiology Laboratory

sop for microbiological assay of erythromycin antibiotic

sop for liquid particle counter

sop for operation and calibration of digital zone reader

sop for monitoring of ultraviolet efficiency LAF and pass box

microbiological assay of cyanocobalamin or vitamin B12

gowning procedure for microbiological testing area

swab testing of various surfaces for bioburden determination

sop for endotoxin challenge test

Hold time study protocol for sterilized media

sop for personnel Qualification protocol for aseptic area

sop for sampling and testing of drain water

Sop for Operation of Airborne Particle Counter

sop for Inoculum Preparation

sop for Validation protocol of steam sterilizer autoclave

sop for pathogen detection from drain point

Sop for Analysis of Raw water Purified water water for injection and pure steam water

sop for preservatives efficacy test

sop for collection and preservation of in house isolated microorganisms

sop for Operation Calibration and Maintenance of Micropipette

Sop for UV Efficacy Test

sop for gram staining

Sop for swab testing

sop for microbiological testing of water

PROCEDURE FOR FUMIGATION

sop for depyrogenation of apparatus

sop for media preparation

sop for fertility test growth promotion test of media

sop for Operation and cleaning of moist heat sterilizer

sop for monitoring by active air sampler

sop for swab sampling and testing for clean rooms in production area

sop for monitoring in microbiology laboratory

sop for Fumigation of aseptic area and microbiology lab

sop for monitoring of personnel in aseptic area

sop for maintenance of cultures

sop for Operation and cleaning of laminar bench

preparation of settle plates

sop for monitoring of pure steam

sop for entry and exit procedure to m.l.t and b.e.t room

sop for storage of and use of media

sop for disposal of microbiological media and cleaning of microbiological glassware

 

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