sop for Receipt Storage and Usage of Culture Media
1.0 OBJECTIVE
1.1 To lay down a procedure for Media Preparation and Consumption.
2.0 SCOPE
2.1 This procedure applicable for Media Preparation and consumption In Microbiology Laboratory
3.0 RESPONSIBILITY
3.1 Microbiologist QC
4.0 ACCOUNTABILITY
4.1 QC Manager
5.0 PROCEDURE
5.1 Requirement
5.1.1 Calibrated balance
5.1.2 Clean Spatula and Measuring cylinder
5.1.2 Non-absorbent cotton
5.1.3 Calibrated pH Meter
5.1.4 Hot Plate
5.1.5 Autoclave
5.1.6 Beaker & Conical Flask
5.1.7 Dehydrated culture media
5.2 Methodology
5.3 The dehydrated culture media as well as their ingredients are highly hygroscopic and must be stored in a cool dry place away from bright light.
5.3.1 Carefully read the instructions printed on the label of dehydrated culture media containers. Check the composition of the medium and the direction for its use.
5.3.2 Examine the physical nature of the dehydrated medium. If any unusual color, odor or physical appearance is noticed, discard the medium.
5.3.3 Always use a dry spoon or spatula for weighing the dehydrated media. The weighing operation shall be completed as quickly
as possible to prevent absorption of moisture by the hygroscopic contents. Wear hand gloves & nose mask while weighing the
dehydrated media to avoid inhalation of fine particles of media.
5.3.4 Operate the balance and weigh accurately the required quantity of dehydrated culture medium on a butter paper transfer the powder to a suitable container.
5.3.5 Add a part of the required quantity of Purified Water and mix the contents. Make up the volume with Purified Water & mix well.
5.3.6 Check the pH of media at 25ºC±2ºC. If there is requirement of pH adjustment, adjust the pH with 1M NaOH or 1M HCl.
5.3.7 Now heat the contents with frequent agitation till the contents are homogenous.
5.3.8 Dispense the reconstituted medium in suitable containers like test tubes, bottles or flasks. The volume of these containers shall be sufficiently
large to facilitate proper aeration and avoid overflow of medium during sterilization.
5.3.9 All culture media containers shall be plugged with non absorbent cotton and covered with a butter paper/aluminum foil.
5.3.10 All the media tubes and flasks should be labeled with details like Name of Media, Media Lot No., Date of Preparation.
5.3.11 Media after preparation and dispensing as above shall be sterilized as soon as possible. Media prepared and dispensed as
above shall be sterilized in an autoclave at 121°C/115°C for 15 minutes.
5.3.12 Specified limits as directed on label of media bottle, discard the media as per SOP for destruction of microbial waste and contaminated media by autoclave SOP.
5.3.13 If the medium is not used in the same day of preparation, it should be stored properly for not more than 7 days.
5.3.14 Stored Media showing any change in appearance or any sign of visible microbial contamination or cracks or dehydration shall be discarded.
5.3.15 Enter details of prepared media in the ‘Media Preparation & Consumption Record’
6.0 ABBREVIATIONS
| Abbreviation used | Full form of abbreviation used |
| SOP | Standard Operating Procedure |
| IPA | Iso Propyl Alcohol |
| QA | Quality assurance |
| SCDM | Soyabean Casein Digest Medium |
| IP | Indian Pharmacopeia |
sop for calibration and validation of micro autoclave
sop for Sterility failure investigation
cleaning and operation of discard autoclave
sop for operation of fogger machine
sop for Biological assay of lactic acid bacillus
sop for preparation of culture inoculum
STP for sterility testing of sterile gloves
sop for Operation and calibration of active air sampler
sop for transfer of material for testing and sampling in sterile area
entry & exit procedure in microbiology laboratory
Growth Promotion Test In Microbiology Laboratory
Operation of B.O.D in Microbiology Laboratory
Operation of Horizontal Laminar Air Flow in the microbiology laboratory
Operation and cleaning of Pass Box.
Operation and cleaning of air sampler
Cleaning and Sterilization of Glassware
Analysis of water for microbial load in microbiology lab
Operation and temperature monitoring of Refrigerator
Fumigation of Microbiology Laboratory.
Entry & Exit procedure In Sterility Area
SOP for Microbial analysis of Raw Material Finished Products
SOP for Operation & Calibration of pH meter in Micro Department
SOP for Operation & Calibration of pH meter in Micro Department
SOP Operation and calibration of Hot Air Oven In Microbiology
SOP for operation cleaning & calibration of Digital colony counter
SOP for Operation And Cleaning of Microscope
sop for Media Preparation and Consumption
sop for Receipt Storage and Usage of Culture Media
sop for Cleaning Sanitization And Disinfection In Microbiology
sop for Environmental monitoring of all the Classified area
sop for Handling and Sub culturing of Microbial cultures
sop for Media Growth Promotion Test and various Microbiological test
sop for BOD incubator operation and cleaning
sop sampling of water for microbiological analysis
sop for Disinfectant Efficacy Test
sop for for cleaning and operation of vortex mixture
sop for Temperature & Relative Humidity Monitoring
sop for Operation and Calibration of Heating Block
sop for Sterility Testing of Microbiology
sop for Disposal of Culture Media
sop for Drain point of Microbiology
sop for entry & exit procedure In Microbial limit test and Biosafety
sop for Gram Staining of Bacteria in Microbiology Laboratory
sop for Monitoring of Compressed Air/gases for microbiological analysis
sop for BET (Bacterial Endotoxin) test in Microbiology
sop for receipt storage and Determining the population of Biological indicators
sop for qualification of analyst microbiologist
sop for Bioburden test of Packing materials in Microbiology Laboratory
sop for microbiological assay of erythromycin antibiotic
sop for liquid particle counter
sop for operation and calibration of digital zone reader
sop for monitoring of ultraviolet efficiency LAF and pass box
microbiological assay of cyanocobalamin or vitamin B12
gowning procedure for microbiological testing area
swab testing of various surfaces for bioburden determination
sop for endotoxin challenge test
Hold time study protocol for sterilized media
sop for personnel Qualification protocol for aseptic area
sop for sampling and testing of drain water
Sop for Operation of Airborne Particle Counter
sop for Validation protocol of steam sterilizer autoclave
sop for pathogen detection from drain point
Sop for Analysis of Raw water Purified water water for injection and pure steam water
sop for preservatives efficacy test
sop for collection and preservation of in house isolated microorganisms
sop for Operation Calibration and Maintenance of Micropipette
sop for microbiological testing of water
sop for depyrogenation of apparatus
sop for fertility test growth promotion test of media
sop for Operation and cleaning of moist heat sterilizer
sop for monitoring by active air sampler
sop for swab sampling and testing for clean rooms in production area
sop for monitoring in microbiology laboratory
sop for Fumigation of aseptic area and microbiology lab
sop for monitoring of personnel in aseptic area
sop for maintenance of cultures
sop for Operation and cleaning of laminar bench
sop for monitoring of pure steam
sop for entry and exit procedure to m.l.t and b.e.t room
sop for storage of and use of media
sop for disposal of microbiological media and cleaning of microbiological glassware