gowning procedure for microbiological testing area

1.0 OBJECTIVE
1.1 The objective of this SOP is to define the procedure for gowning as well as degowning of any sterile gown employed for aseptic areas.

2.0 SCOPE
2.1 This SOP is applicable for for gowning as well as degowning of any sterile gown employed for aseptic areas
3.0 RESPONSIBILITY
3.1 Microbiologist-Quality control- To follow the SOP for gowning as well as degowning of any sterile gown employed for aseptic areas.
3.2 Head- Quality Control – Training on SOP.
3.3 Head- Quality Control- Implementation & follow up of SOP.
4.0 ACCOUNTABILITY
4.1 Head – Quality Control
5.0 REFERENCE(S)
5.1 In-House

6.0 PROCEDURE
6.1 GOWNING
6.1.1 Remove all street clothing, factory uniforms and hang properly inside the SS dress cabinet on SS dress hanger kept in the IST Change room.

Also remove all ornaments, wrist watches and keep them properly inside the SS dress cabinet.
6.1.2 Disinfect both the hands up to elbow with the 70% IPA solution kept in the IST Change Room. Remove dirt from both the feet

by stepping over the tacky mat placed before entering the IInd change room.
6.1.3 Enter the IInd Change room, by opening the door of the IInd Change room with the help of elbow.
6.1.4 First wear a pair of sterilized gloves in both hands. Now take out sterile gown wrapped in butter paper/parchment Paper from garment cabinet.
6.1.5 Wear sterile facemask and then wear the headgear. Tie the headgear properly such that all hairs should be tucked inside the headgear.
6.1.6 Next, wear the boiler suit carefully by holding the boiler suit by two ends and crimp it. Now slowly slip one of the legs inside the suit in such a

way that the suit should not touch the floor or wall of the room. Then slip the other leg similarly by taking care.
6.1.7 Next, slip both hands properly inside the boiler suit carefully by stretching both the hands. Tuck the Headgear properly inside the boiler suit.
6.1.8 Sit on the SS Crossover bench and wear the booties properly for one leg. Move the leg to other side of the cross over bench and then follow

the same procedure for the other leg. Tuck the leg portion of boiler suit inside the booties properly.
6.1.9 Cross the SS Crossover bench and move to other side of the room in a sitting position. Wear cleaned eyeglass properly.
6.1.10 Now wear second pair of sterilized hand gloves over the existing hand gloves pair and then tuck the cuff of boiler suit properly inside the hand gloves for both the hands.
6.1.11 Rinse both the hand gloves with 70% filtered IPA solution kept at IInd Change room .
6.1.12 Checking of final gowning procedure shall be done in front of the mirror attached in the IInd Change room. Move inside the clean corridor by opening the with the help of elbow.

6.2 DEGOWNING
6.2.1 After completing the work inside the sterile analysis area, move into the clean corridor by opening the door. Pull open the doors of the Exit Air Lock 1st.
6.2.2 Remove carefully and slowly the booties, boiler suit and the head gear in the and dispose the dress properly inside Closed SS container for washing.
6.2.3 Dispose the used hand gloves as well as facemask in a separate SS closed disposal bin.
6.2.4 Wear the factory uniform, wristwatch and ornaments by taking it out from the SS dress Cabinet properly and slowly exit from the IST Change room.
6.2.5 Keep the eyeglasses inside the SS dress cabinet and reuse the eyeglasses after moping and drying properly with filtered disinfectant solution, immediately before gowning operations.

7.0 ABBREVIATIONS
SOP : Standard Operating Procedure
QAD : Quality Assurance Department
QCD : Quality Control Department
IPA : Isoproply alcohol
NA : Not Applicable
No. : Number

gowning procedure for microbiological testing area

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