Operation and calibration of Conductivity Meter
1.0 Objective
To lay down a procedure for Operation and Calibration of Conductivity Meter.
2.0 Scope
This Standard Operating Procedure is applicable for the Operation and Calibration of Conductivity Meter in Quality control department to be followed at formulation plants of abc company.
3.0 Responsibility
3.1 Officer/Executive QC or his designee shall be responsible to followed the SOP
3.2 Head QC or his designee shall be responsible to ensure the activity as per SOP.
3.3 Head QA or his designee shall be responsible for implementation and compliance with SOP.
4.0 Abbreviations and Definitions
SOP : Standard Operating Procedure
QA : Quality Assurance
QC : Quality Control
No. : Number
ml : Milliliter
% : Percentage
ºC : Degree Centigrade
5.0 Procedure
5.1 Operation
5.1.1 Clean the Conductivity electrode with purified water.
5.1.2 Switch “ON” the main power supply.
5.1.3 Press “MODE” Key, the display will shows Conductivity and select measurement mode Conductivity.
5.1.4 Press”2” Key and than “ENTER” Key to select the Conductivity mode.
5.1.5 Enter the cell constant 0.1 of the connected cell (electrode) than display will be show calibrate: i) Y, ii) N.
5.1.6 To continue the operation press “2”.
5.1.7 Instrument get stabilize itself with message on the screen “Wait stabilizing” which shows
the measurement Conductivity and temperature.
5.1.8 Select the temperature mode (automatic or manual) for automatic temperature compensation
is to be considered during measurement at 20 to 25ºC whichever select for manual temperature
compensation, measure the solution temperature with external thermometer and then enter the correct
value if necessary.
5.1.9 Place the electrode and temperature sensor in “Sample Solution”.
5.1.10 Note down the reading direct from the display.
5.1.11 Lift the electrode with temperature sensor and rinse with purified water 2-3 times.
5.1.12 At the ends of shift “OFF” the instrument and main power supply.
5.2 Calibration (Preparation of KCl solution )
Frequency: Daily
5.2.1 Prepare standard potassium chloride solutions 0.001M as follows:
5.2.2 0.1 M KCl Solution: Transfer about 3.7265g of potassium chloride accurately weight to 500 ml
volumetric flask, dissolve in purified water and make up volume up to the mark.
5.2.3 This solution should be used within one month from the date of preparation, store the at
room temperature.
5.2.4 0.01M KCl Solution: Pipette 20ml of standard KCl solution (0.1M) to 200 ml flask and make
up to the mark with purified water.
5.2.5 0.001 M KCl solution: Pipette 20ml of standard KCl solution (0.01M) to 200 ml flask and make up
to the mark with purified water.
Note: Dilute 0.1M KCL solution to required concentration before adjusting the conductivity meter,
0.001 M KCl solution use within 7 days from the date of preparation. Store the solution at room temperature
in properly closed flask.
5.3 Procedure for Cell Constant Check
5.3.1 Follow operation procedure as per above.
5.3.2 Enter standard solution conductivity 147 µS.
5.3.3 Select Temp. Co-efficient value.
5.3.4 Observed reading and press Enter if stable.
5.3.5 If reading is within limit, the instrument will calibrate the electrode and display the conductivity.
5.3.6 Remove and wash the electrode with purified water and keep the electrode in purified water.
5.3.7 Acceptance Criteria: The observed cell constant for 0.001MKCL solution shall be as mentioned below.
| Actual Cell | Cell Constant |
| 0.1 | 0.080 to 0.120 |
Calibration of Conductivity meter and Conductivity Cell
5.4.1 Prepare standard potassium chloride solutions 0.001 M as follows:
5.4.2 0.001 M KCL solution: Prepare as per point No. 5.2.2 to 5.2.5.
5.4.3 Cleaned the conductivity electrode with purified water.
5.4.4 Place the electrode and temperature sensor in 0.001M KCl solution.
5.4.5 Press TEMPCO Key and enter appropriate Co-efficient value.
5.4.6 Press enter Key and instrument will display Conductivity.
5.4.7 Remove and wash the electrode with purified water and keep the electrode in purified water.
5.4.8 Acceptance Criteria: The observed conductivity of standard KCl solution shall be mention below:
| Concentration of standard KCl solution | Conductivity Limit |
| 0.001 M | 147 + 2.94 µS/cm |
6.0 Forms and Records
6.1 Calibration Record of Conductivity meter – Annexure-1
7.0 Distribution
7.1 Master copy – Documentation Cell (Quality Assurance)
7.2 Control copy – Quality Control
8.0 History
| Revision Number | Details For Change |
Reason for Revision |
| 00 | New SOP | NA |
cleaning and operation of discard autoclave
sop for operation of fogger machine
sop for Biological assay of lactic acid bacillus
sop for preparation of culture inoculum
STP for sterility testing of sterile gloves
sop for Operation and calibration of active air sampler
sop for transfer of material for testing and sampling in sterile area
entry & exit procedure in microbiology laboratory
Growth Promotion Test In Microbiology Laboratory
Operation of B.O.D in Microbiology Laboratory
Operation of Horizontal Laminar Air Flow in the microbiology laboratory
Operation and cleaning of Pass Box.
Operation and cleaning of air sampler
Cleaning and Sterilization of Glassware
Analysis of water for microbial load in microbiology lab
Operation and temperature monitoring of Refrigerator
Fumigation of Microbiology Laboratory.
Entry & Exit procedure In Sterility Area
SOP for Microbial analysis of Raw Material Finished Products
SOP for Operation & Calibration of pH meter in Micro Department
SOP for Operation & Calibration of pH meter in Micro Department
SOP Operation and calibration of Hot Air Oven In Microbiology
SOP for operation cleaning & calibration of Digital colony counter
SOP for Operation And Cleaning of Microscope
sop for Media Preparation and Consumption
sop for Receipt Storage and Usage of Culture Media
sop for Cleaning Sanitization And Disinfection In Microbiology
sop for Environmental monitoring of all the Classified area
sop for Handling and Sub culturing of Microbial cultures
sop for Media Growth Promotion Test and various Microbiological test
sop for BOD incubator operation and cleaning
sop sampling of water for microbiological analysis
sop for Disinfectant Efficacy Test
sop for for cleaning and operation of vortex mixture
sop for Temperature & Relative Humidity Monitoring
sop for Operation and Calibration of Heating Block
sop for Sterility Testing of Microbiology
sop for Disposal of Culture Media
sop for Drain point of Microbiology
sop for entry & exit procedure In Microbial limit test and Biosafety
sop for Gram Staining of Bacteria in Microbiology Laboratory
sop for Monitoring of Compressed Air/gases for microbiological analysis
sop for BET (Bacterial Endotoxin) test in Microbiology
sop for receipt storage and Determining the population of Biological indicators
sop for qualification of analyst microbiologist
sop for Bioburden test of Packing materials in Microbiology Laboratory
sop for microbiological assay of erythromycin antibiotic
sop for liquid particle counter
sop for operation and calibration of digital zone reader
sop for monitoring of ultraviolet efficiency LAF and pass box
microbiological assay of cyanocobalamin or vitamin B12
gowning procedure for microbiological testing area
swab testing of various surfaces for bioburden determination
sop for endotoxin challenge test
Hold time study protocol for sterilized media
sop for personnel Qualification protocol for aseptic area
sop for sampling and testing of drain water
Sop for Operation of Airborne Particle Counter
sop for Validation protocol of steam sterilizer autoclave
sop for pathogen detection from drain point
Sop for Analysis of Raw water Purified water water for injection and pure steam water
sop for preservatives efficacy test
sop for collection and preservation of in house isolated microorganisms
sop for Operation Calibration and Maintenance of Micropipette
sop for microbiological testing of water
sop for depyrogenation of apparatus
sop for fertility test growth promotion test of media
sop for Operation and cleaning of moist heat sterilizer
sop for monitoring by active air sampler
sop for swab sampling and testing for clean rooms in production area
sop for monitoring in microbiology laboratory
sop for Fumigation of aseptic area and microbiology lab
sop for monitoring of personnel in aseptic area
sop for maintenance of cultures
sop for Operation and cleaning of laminar bench
sop for monitoring of pure steam
sop for entry and exit procedure to m.l.t and b.e.t room
sop for storage of and use of media
sop for disposal of microbiological media and cleaning of microbiological glassware
Preparation Filtration Usage and Destruction of Disinfectant Solution
Entry Exit and gowning Procedure in Microbiology Laboratory
Safety measures to be followed in microbiology laboratory
Growth Promotion Test of Media
Preservation and Maintenance of Microbial Strain
Entry and Exit in Microbiology Sterile Area
Entry and Exit in Microbiology Limit testing area
Water sampling of raw water purified water WFI and PSG in microbiology Lab
Microbial monitoring of Drain points
sop for Operation of Double Door Autoclave
Operation and Calibration of Thermo block
Operation and Calibration of Micro pipette
Operation and calibration of BOD-Incubators
Operation and Calibration of Microscope
Operation and Calibration of Air Sampler
Disinfectant Preparation and Cleaning of Microbiology Lab
Operation and cleaning of Static Pass Box