Operation and Calibration of Air Sampler
1.0 Objective
To lay down a procedure for Operation and calibration of Air sampler.
2.0 Scope
This Standard Operating Procedure at Microbiology laboratory Quality control department of abc company.
3.0 Responsibility
3.1 Concerned Officer/Executive shall be responsible to follow this SOP.
3.2 Department Head or his designee shall be responsible to implement and ensuring to compliance of this SOP.
4.0 Abbreviations and Definitions
SOP : Standard Operating Procedure
QC : Quality Control
QA : Quality Assurance
SD : Standard deviation
Cal : Calibration
5.0 Procedure
5.1 Operation and calibration
5.1.1 Connect the power cable to instrument and power supply, Recharge the instrument for an one hour before for every usage.
5.1.2 Switch ON the instrument by once single pressing on the ‘ON/OFF’ button.
5.1.3 Set the time for 1000 liters by using ‘Set’ and ‘Arrow’ buttons.
5.1.4 Carry the sterile SS stain cup and Petri plates in a sterile condition to area where the Air sampling is scheduled.
5.1.5 Open the SS stain cup, and assemble the Petri plate in a direction to open side of air flow.
5.1.6 Prepare the media and Petri plate.
5.1.7 Once the Petri plates are assembled, press the start button.
5.1.8 After 1000 liters completed air vacuum pump will gets stops automatically.
5.1.9 Open the SS stain cup and remove the Petri plate from the instrument, close the Petri plate with upper part of Petri plate immediately.
5.1.10 Carry the Petri plate in a sterile condition to the Microbiology lab.
5.1.11 Keep the plates for incubation as per respective SOP No BBL/QC/SOP/028.
5.2 Cleaning and Sterilization
5.2.1 Before using the air sampler, wrapped SS stain cup in butter paper and keep it in Autoclave for Sterilization.
5.2.2 Sanitize the tubular metallic handle with 70 % v/v Isopropyl alcohol.
5.2.3 Clean the impeller with 70 % v/v Isopropyl alcohol between sampling cycles.
5.3 Precautions
5.3.1 Do not autoclave the tubular stainless body, it will damage the system.
5.3.2 Ensure the battery power is sufficient for conducting the test well in advance.
5.3.3 Test the power fall of the battery by switching on the control box and note the alarm, which should occur at any given timer selection.
5.3.4 For the charging the battery, bring the DC/AC switch on the neutral position and connect to 220V AC mains, using power cord.
5.3.5 After completion of the work put the air sampler into its carry box.
5.3.6 Calibration shall be performed for Motor /Battery efficiency, and for Air flow rate with calibrated anemometer by authorized out source calibration party once a year and when ever a major maintenance is done.
5.3.7 Acceptance criteria: 180 liters air per minute.
6.0 Forms and Records
6.1 Air Sampler-Usage log book – Annexure-1
7.0 Distribution
7.1 Master copy – Documentation Cell (Quality Assurance)
7.2 Control copy – Quality Control
8.0 History
| Revision Number | Details For Change |
Reason for Revision |
| 00 | New SOP | NA |
sop for calibration and validation of micro autoclave
sop for Sterility failure investigation
cleaning and operation of discard autoclave
sop for operation of fogger machine
sop for Biological assay of lactic acid bacillus
sop for preparation of culture inoculum
STP for sterility testing of sterile gloves
sop for Operation and calibration of active air sampler
sop for transfer of material for testing and sampling in sterile area
entry & exit procedure in microbiology laboratory
Growth Promotion Test In Microbiology Laboratory
Operation of B.O.D in Microbiology Laboratory
Operation of Horizontal Laminar Air Flow in the microbiology laboratory
Operation and cleaning of Pass Box.
Operation and cleaning of air sampler
Cleaning and Sterilization of Glassware
Analysis of water for microbial load in microbiology lab
Operation and temperature monitoring of Refrigerator
Fumigation of Microbiology Laboratory.
Entry & Exit procedure In Sterility Area
SOP for Microbial analysis of Raw Material Finished Products
SOP for Operation & Calibration of pH meter in Micro Department
SOP for Operation & Calibration of pH meter in Micro Department
SOP Operation and calibration of Hot Air Oven In Microbiology
SOP for operation cleaning & calibration of Digital colony counter
SOP for Operation And Cleaning of Microscope
sop for Media Preparation and Consumption
sop for Receipt Storage and Usage of Culture Media
sop for Cleaning Sanitization And Disinfection In Microbiology
sop for Environmental monitoring of all the Classified area
sop for Handling and Sub culturing of Microbial cultures
sop for Media Growth Promotion Test and various Microbiological test
sop for BOD incubator operation and cleaning
sop sampling of water for microbiological analysis
sop for Disinfectant Efficacy Test
sop for for cleaning and operation of vortex mixture
sop for Temperature & Relative Humidity Monitoring
sop for Operation and Calibration of Heating Block
sop for Sterility Testing of Microbiology
sop for Disposal of Culture Media
sop for Drain point of Microbiology
sop for entry & exit procedure In Microbial limit test and Biosafety
sop for Gram Staining of Bacteria in Microbiology Laboratory
sop for Monitoring of Compressed Air/gases for microbiological analysis
sop for BET (Bacterial Endotoxin) test in Microbiology
sop for receipt storage and Determining the population of Biological indicators
sop for qualification of analyst microbiologist
sop for Bioburden test of Packing materials in Microbiology Laboratory
sop for microbiological assay of erythromycin antibiotic
sop for liquid particle counter
sop for operation and calibration of digital zone reader
sop for monitoring of ultraviolet efficiency LAF and pass box
microbiological assay of cyanocobalamin or vitamin B12
gowning procedure for microbiological testing area
swab testing of various surfaces for bioburden determination
sop for endotoxin challenge test
Hold time study protocol for sterilized media
sop for personnel Qualification protocol for aseptic area
sop for sampling and testing of drain water
Sop for Operation of Airborne Particle Counter
sop for Validation protocol of steam sterilizer autoclave
sop for pathogen detection from drain point
Sop for Analysis of Raw water Purified water water for injection and pure steam water
sop for preservatives efficacy test
sop for collection and preservation of in house isolated microorganisms
sop for Operation Calibration and Maintenance of Micropipette
sop for microbiological testing of water
sop for depyrogenation of apparatus
sop for fertility test growth promotion test of media
sop for Operation and cleaning of moist heat sterilizer
sop for monitoring by active air sampler
sop for swab sampling and testing for clean rooms in production area
sop for monitoring in microbiology laboratory
sop for Fumigation of aseptic area and microbiology lab
sop for monitoring of personnel in aseptic area
sop for maintenance of cultures
sop for Operation and cleaning of laminar bench
sop for monitoring of pure steam
sop for entry and exit procedure to m.l.t and b.e.t room
sop for storage of and use of media
sop for disposal of microbiological media and cleaning of microbiological glassware
Preparation Filtration Usage and Destruction of Disinfectant Solution
Entry Exit and gowning Procedure in Microbiology Laboratory
Safety measures to be followed in microbiology laboratory
Growth Promotion Test of Media
Preservation and Maintenance of Microbial Strain
Entry and Exit in Microbiology Sterile Area
Entry and Exit in Microbiology Limit testing area
Water sampling of raw water purified water WFI and PSG in microbiology Lab
Microbial monitoring of Drain points
sop for Operation of Double Door Autoclave
Operation and Calibration of Thermo block
Operation and Calibration of Micro pipette
Operation and calibration of BOD-Incubators
Operation and Calibration of Microscope
Operation and Calibration of Air Sampler