sop for sampling and testing of drain water

1.0 OBJECTIVE
1.1 The objective of this SOP is to define the procedure for sampling (before sanitization and after sanitization) and testing of drain water.
2.0 SCOPE
2.1 This SOP is applicable for sampling for sampling (before sanitization and after sanitization) and testing of drain water and Microbiological Analysis
3.0 RESPONSIBILITY
3.1 Microbiologist-Quality control- To follow the SOP sampling for sampling (before sanitization and after sanitization) and testing of drain water for Microbiological Analysis.
3.2 Head- Quality Control – Training on SOP.
3.3 Head- Quality Control- Implementation & follow up of SOP.
4.0 ACCOUNTABILITY
4.1 Head – Quality Control

5.0 REFERENCE(S)
5.1 In-House

6.0 ENVIRONMENT, HEALTH AND SAFETY
6.1 As per procedure.

7.0 PROCEDURE
7.1 Collect approx 20 ml of drain sample (Before and after sanitization) in sterile container from
the drainage using sterile pipette.
7.2 Use sucking bulb and Pipette for collection of sample.
7.3 Testing Procedure for TOTAL VIABLE COUNT
7.4 Take 1 ml sample and dilute to 9 ml with sterile peptone water (1:10 dilution )
7.5 Take 1 ml from 1:10 dilution & further dilute to 9 ml with sterile sterile peptone water
7.6 (1:100 dilution). Similarly prepare 1:1000 dilution.
7.7 Finally take 1 ml sample solution from 1: 100 dilution or 1:1000 dilution with the help of
7.8 sterile micro Recent Updates
7.9 pipette in to sterile Petri dish aseptically for Bacteria and fungi count.
7.10 Perform the test in duplicate.
7.11 Pour approximately 20 ml of Sayabean Casein digest agar for bacteria and Sabouraud dextrose
7.12 Fungi previously melted and cool to 45 0c in to two different plates and mix well by tilting
rotating. Allow solidifying.
7.13 Write down the location No. and date an each plate.
7.14 Incubate the SCD agar plate at 30-35°C for bacteria for 3 days and Sabouraud dextrose agar
plate at 20-25°C for fungi for 5 days
7.15 After incubation period, examine the plate far growth . (No. of colonies)
7.16 Calculate the average colonies of two plates in terms of cfu/ml.
7.17 Count the No. of colonies as per following calculation and record in Annexure I
7.18 Calculation
7.18.1 1 : 100 a If the plate show N No. of cfu, the No. of cfu/ml = N x 100
7.18.2 1 : 1000 a If the plate show N No. of clu, the No. of = N x 1000
7.18.3 Pathogen Testing as per SOP and Record the results in
Annexure – I
7.19 Frequency: Once in a month.

8.0 ABBREVIATIONS
SOP : Standard Operating Procedure
QAD : Quality Assurance Department
QCD : Quality Control Department
IPA : Isoproply alcohol
NA : Not Applicable
No. : Number

9.0 ANNEXURE

ANNEXURE NO.	Title of Annexure	Format No. of Annexure
ANNEXURE I	Microbial Monitoring Of Drain Point
ANNEXURE II	Sampling Plan For Drain Point

S. No.	Type of Water	Sampling Point No.	Location	Frequency
1.	Drain Water	DW-01	Media Preparation Room (Micro Lab)	Once in a month
2.	Drain Water	DW-02	Autoclave Room (Micro Lab)
3.	Drain Water	DW-03	Discarding Room (Micro Lab)
4.	Drain Water	DW-04	Chemical Room
5.	Drain Water	DW-05	Production Autoclave Room
6.	Drain Water	DW-06
7.	Drain Water	DW-07
8.	Drain Water	DW-08
9.	Drain Water	DW-09
10.	Drain Water	DW-10
11.	Drain Water	DW-11
12.	Drain Water	DW-12
13.	Drain Water	DW-13
14.	Drain Water	DW-14
15.	Drain Water	DW-15
Frequency:- Once in a month

sop for sampling and testing of drain water

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